Recombinant Human TLR6 Fc Chimera Protein, CF

Catalog #: 7755-TR
1 Citation Datasheet / COA / SDS

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7755-TR-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TLR6 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When peptidoglycan is immobilized at 10 µg/mL (100 µL/well), the concentration of Recombinant Human TLR6 Fc Chimera that produces 50% of the optimal binding response is approximately 0.05‑0.25 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TLR6 protein

Human TLR6 (Asn32-Asn583) Accession # AAY88759	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAY88759

N-terminal Sequence
Analysis

Asn32

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

89.9 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Product Datasheets

7755-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7755-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TLR6

The Toll‑like family of molecules serve as pattern recognition receptors that activate the immune system in response to microbial pathogens. Toll‑like receptors (TLRs) are type I transmembrane proteins in either the plasma membrane or the membranes of endosomal structures. TLRs contain a large number of leucine‑rich repeats (LRRs) in their extracellular domains (ECD) and a cytoplasmic tail with one signal transducing Toll/IL‑1 receptor (TIR) domain (1, 2). Mature human TLR6 is an approximately 115 kDa protein that consists of a 555 amino acid (aa) ECD, a 21 aa transmembrane segment, and a 189 aa cytoplasmic domain (3). Within the ECD, human TLR6 shares 72% aa sequence identity with mouse and rat TLR6. TLR6 is expressed on the surface of macrophages, monocytes, neutrophils, dermal endothelial cells, and alveolar epithelial cells in ligand‑independent association with TLR2 (4‑7). TLR2 also associates with TLR1, creating a functional receptor complex with specificity for related microbial ligands (4, 8, 9). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycans, and glycosylphosphatidylinositols (4‑6, 10‑13). TLR6 plays an important role in the control of Th17 responses and IL‑23 production during allergic inflammation and fungal‑induced asthma (7).

References

Mills, K.H. (2011) Nat. Rev. Immunol. 11:807.
Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Takeuchi, O. et al. (1999) Gene 231:59.
Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
Nakao, Y. et al. (2005) J. Immunol. 174:1566.
Bulut, Y. et al. (2001) J. Immunol. 167:987.
Moreira, A.P. et al. (2011) J. Clin. Invest. 121:4420.
Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.

Long Name

Toll-like Receptor 6

Entrez Gene IDs

10333 (Human); 21899 (Mouse); 305353 (Rat)

Alternate Names

CD286 antigen; CD286; TLR6; toll-like receptor 6

Related Research Areas

Citation for Recombinant Human TLR6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation
Authors: C Mayslich, PA Grange, M Castela, AG Marcelin, V Calvez, N Dupin
International Journal of Molecular Sciences, 2022-05-03;23(9):.
Species: Human
Sample Types: Protein
Applications: Bioassay, Western Blot (Detection)