Recombinant Human TLR4 Protein, CF

Name: Recombinant Human TLR4 Protein, CF
Brand: R&D Systems
SKU: 1478-TR
Rating: 5 (2 reviews)

Catalog #: 1478-TR
15 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1478-TR-050

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Citations (15)

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Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TLR4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human TLR4 protein
Glu24-Lys631, with a C-terminal Ser and 10-His tag

Accession #

O00206

N-terminal Sequence
Analysis

Glu24

Predicted Molecular Mass

70.6 kDa

SDS-PAGE

90-95 kDa, reducing conditions

Product Datasheets

1478-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1478-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll‑Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD‑2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL‑1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide‑linked homo‑oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

References

Miyake, K. (2004) Semin. Immunol. 16:11.
Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
Erridge, C. (2010) J. Leukoc. Biol. 87:989.
Medzhitov, R. et al. (1997) Nature 388:394.
Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
Akashi, S. et al. (2000) J. Immunol. 164:3471.
Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Related Research Areas

Citations for Recombinant Human TLR4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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The bacterial lysate OM-85 engages Toll-like receptors 2 and 4 triggering an immunomodulatory gene signature in human myeloid cells
Authors: Khameneh, HJ;Bolis, M;Ventura, PMO;Cassanmagnago, GA;Fischer, BA;Zenobi, A;Guerra, J;Buzzago, I;Bernasconi, M;Zaman, GJR;Rinaldi, A;Moro, SG;Sallusto, F;Baulier, E;Pasquali, C;Guarda, G;
Mucosal immunology
Species: N/A
Sample Types: Small Molecule
Applications: Bioassay
The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice
Authors: J Zhang, Y Sun, C Sun, D Shang
Bioorganic chemistry, 2023-01-18;132(0):106376.
Species: N/A
Sample Types: Peptide
Applications: Surface Plasmon Resonance
Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation
Authors: C Mayslich, PA Grange, M Castela, AG Marcelin, V Calvez, N Dupin
International Journal of Molecular Sciences, 2022-05-03;23(9):.
Species: Human
Sample Types: Protein
Applications: Bioassay, Western Blot (Detection)
Neuron-Microglia Contacts Govern the PGE2 Tolerance through TLR4-Mediated de Novo Protein Synthesis
Authors: HC Kuo, KF Lee, SL Chen, SC Chiu, LY Lee, WP Chen, CC Chen, CH Chu
Biomedicines, 2022-02-10;10(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
LYSMD3: A mammalian pattern recognition receptor for chitin
Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
Cell Reports, 2021-07-20;36(3):109392.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Astilbin prevents osteoarthritis development through the TLR4/MD-2 pathway
Authors: S Sun, Z Yan, X Shui, W Qi, Y Chen, X Xu, Y Hu, W Guo, P Shang
J Cell Mol Med, 2020-10-16;0(0):.
Species: Human
Sample Types: Peptide
Applications: ELISA Capture
Toll-like receptor 4-mediated inflammation triggered by extracellular IFI16 is enhanced by lipopolysaccharide binding
Authors: A Iannucci, V Caneparo, S Raviola, I Debernardi, D Colangelo, R Miggiano, G Griffante, S Landolfo, M Gariglio, M De Andrea
PLoS Pathog., 2020-09-09;16(9):e1008811.
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance
Procyanidin B3 alleviates intervertebral disc degeneration via interaction with the TLR4/MD-2 complex
Authors: P Shang, Q Tang, Z Hu, S Huang, Y Hu, J Zhu, H Liu
J. Cell. Mol. Med., 2020-02-18;0(0):.
Applications: ELISA (Standard)
Inorganic polyphosphate potentiates lipopolysaccharide-induced macrophage inflammatory response
Authors: T Ito, S Yamamoto, K Yamaguchi, M Sato, Y Kaneko, S Goto, Y Goto, I Narita
J. Biol. Chem., 2020-02-10;0(0):.
Species: N/A
Sample Types: Complex Sample Type
Applications: Bioassay
Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin
Authors: M Zheng, A Ambesi, P J McKeown-
Cells, 2020-01-15;9(1):.
Species: Human
Sample Types:
Applications: Cell Culture

Galectin-3 promotes A&beta oligomerization and A&beta toxicity in a mouse model of Alzheimer's disease
Authors: CC Tao, KM Cheng, YL Ma, WL Hsu, YC Chen, JL Fuh, WJ Lee, CC Chao, EHY Lee
Cell Death Differ., 2019-05-24;27(1):192-209.
Species: Human
Sample Types: Hippocampal Cell Lysates
Applications: IP

Identification of FimH derivatives as adjuvant vaccinated with PAc that enhance protection against Streptcoccus mutans colonization
Authors: Z Liu, J Chen, W Li, Y Bi, Y Li, M Fan
Mol. Cell. Probes, 2019-03-30;0(0):.
Species: Bacteria - E. Coli
Sample Types: Recombinant Protein
Applications: Far Western Blot

Specific Interactions Measured by AFM on Living Cells between Peroxiredoxin-5 and TLR4: Relevance for Mechanisms of Innate Immunity
Authors: B Knoops, S Becker, MA Poncin, J Glibert, S Derclaye, A Clippe, D Alsteens
Cell Chem Biol, 2018-03-15;0(0):.

Importance of the C-terminal histidine residues of Helicobacter pylori GroES for Toll-like receptor 4 binding and interleukin-8 cytokine production
Sci Rep, 2016-11-21;6(0):37367.
Applications: Surface Plasmon Resonance

Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcgamma receptors.
Authors: Loyau J, Malinge P, Daubeuf B, Shang L, Elson G, Kosco-Vilbois M, Fischer N, Rousseau F
MAbs, 2014-01-01;6(6):1621-30.
Species: Human
Sample Types: Antibody
Applications: ELISA Developmet