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Recombinant Human Thrombopoietin (NS0-expressed) Protein

Catalog #: 288-TPN
13 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
288-TPN-025/CF
288-TPN-010/CF
288-TPN-100/CF

With Carrier

Catalog # Availability Size / Price Qty
288-TPN-025
Recombinant Human Thrombopoietin (NS0-expressed) Protein Bioactivity
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Product Details
Citations (13)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Thrombopoietin (NS0-expressed) Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 5-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Thrombopoietin/Tpo protein
Ser22-Gly353
Accession #
P40225
N-terminal Sequence
Analysis
Ser22
Predicted Molecular Mass
35 kDa
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

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288-TPN (with carrier)

Product Datasheet
COA
SDS

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288-TPN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

288-TPN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

288-TPN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Thrombopoietin (NS0-expressed) Protein Bioactivity View Larger

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 5-15 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1 - 3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

References
  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995) Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Citations for Recombinant Human Thrombopoietin (NS0-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Reprogramming Megakaryocytes for Controlled Release of Platelet-like Particles Carrying a Single-Chain Thromboxane A2 Receptor-G-Protein Complex with Therapeutic Potential
    Authors: Lu, R;Li, Y;Xu, A;King, B;Ruan, KH;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Improved Sendai viral system for reprogramming to naive pluripotency.
    Authors: Kunitomi A, Hirohata R, Arreola V, Osawa M, Kato T, Nomura M, Kawaguchi J, Hara H, Kusano K, Takashima Y, Takahashi K, Fukuda K, Takasu N, Yamanaka S
    Cell Rep Methods, 2022-10-17;2(11):100317.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.
    Authors: Sudo K, Yamazaki S, Wilkinson A, Nakauchi H, Nakamura Y
    Stem Cell Res, 2021-09-03;56(0):102531.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential.
    Authors: Aerts-Kaya F, Visser T, Pervin B, Mammadova A, Ozyuncu O, Wagemaker G, Uckan-Cetinkaya F
    Biol Blood Marrow Transplant, 2020-01-07;26(4):634-642.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition.
    Authors: Ohta R, Sugimura R, Niwa A, Saito M
    J Vis Exp, 2019-06-16;0(148):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011-07-27;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells.
    Authors: Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF
    Gene Ther., 2011-05-05;18(11):1078-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Applications: ELISA (Standard)
  9. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008-06-17;36(9):1121-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELO-ITGA2.
    Authors: Cheli Y, Kanaji S, Jacquelin B, Chang M, Nugent D, Kunicki T
    Biochim Biophys Acta, 2007-07-06;1769(9):546-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation.
    Authors: Kanaji S, Kanaji T, Jacquelin B, Chang M, Nugent D, Komatsu N, Moroi M, Izuhara K, Kunicki T
    Blood, 2005-02-08;105(10):3888-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, 107325, Miyamoto T, Akashi K, Weissman IL
    Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases, 2002-08-22;99(18):11872-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Why do you sell Thrombopoietin protein from two different expression systems?  Under what circumstances would it be preferred to use the NS0-expressed protein?

    • The NS0-expressed protein is necessary for the serum-free megakaryocyte colony stimulating assay (Nadine Weich, Genetics Insititute).  In all other assays, it works the same as the Sf-21 derived protein.

  2. What are the differences between the Recombinant Human Thrombopoietin/Tpo Proteins (Catalog #s 288-TP, 288-TPN, and 288-TPE)?

    • Each of these proteins is produced using a different expression system. 288-TP is Spodoptera frugiperda, Sf21 baculovirus-derived; 288-TPN is mouse myeloma cell line, NS0-derived; and 288-TPE is E. coli-derived.

      Additionally, the sequence length also varies. 288-TP and 288-TPN begin at Ser22 and end at Gly353. 288-TPE also begins at Ser22 but is truncated at the C-terminus, ending at Leu195. Truncation of the C-terminus has been shown to enhance the biological activity of Tpo. 

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