Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF

Catalog #: AVI3025 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI3025-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TGF-beta RI/ALK-5 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) TGF-beta RII Fc Chimera (Catalog # 341-BR) is immobilized at 1 μg/mL (100 μL/well), in the presence of rhTGF‑ beta 1 (Catalog # 240-B), Biotinylated Recombinant Human TGF‑ beta RI/ALK-5 Fc Chimera Avi-tag protein binds with an ED₅₀ of 0.15‑0.75 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta RI/ALK-5 protein

Human TGF-beta R1/ALK-5 (Ala25 or Leu34-Glu125) Accession # P36897.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P36897.1

N-terminal Sequence
Analysis

Ala25, Leu34

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

39 kDa

SDS-PAGE

43-60 kDa, under reducing conditions

Product Datasheets

AVI3025

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI3025

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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When Recombinant Human TGF-beta RII Fc Chimera (341-BR) is immobilized at 1 μg/mL (100 μL/well), in the presence of Recombinant Human TGF-beta 1 (240-B), it binds Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag (Catalog # AVI3025) with an ED₅₀ of 0.15-0.75 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human TGF-beta RI/ALK-5 Fc Chimera Avi-tag (Catalog # AVI3025) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-60 kDa and 86-120 kDa, respectively

Reconstitution Calculator

Background: TGF-beta RI/ALK-5

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, human TGF-beta RI shares 90% and 88% aa sequence identity with mouse and rat TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9). Our Avi-tg Biotinylated human TGF-beta RI features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Wrana, J.L. et al. (1994) Nature 370:341.
Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
Huse, M. et al. (1999) Cell 96:425.
Marcías-Silva, M. et al. (1996) Cell 87:1215.
Zhang, Y. et al. (1996) Nature 383:168.
Huse, M. et al. (2001) Mol. Cell 8:671.
Larsson, J. et al. (2001) EMBO J. 20:1663.
James, J.M. et al. (2013) Development 140:3903.
Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.

Long Name

Transforming Growth Factor beta Receptor I

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Alternate Names

AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I