Recombinant Human TGF-beta 3, Animal-Free Protein

Catalog #: Qk054 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
Qk054-0050
Qk054-0500

Recombinant Human TGF-beta 3, Animal-Free Protein Bioactivity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TGF-beta 3, Animal-Free Protein Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

No significant difference between EC₅₀ of reference and test lots

Source

E. coli-derived human TGF-beta 3 protein

Accession #

P10600.1

Predicted Molecular Mass

12.7 kDa (monomer), 25.4 kDa (dimer)

SDS-PAGE

Dimeric TGF- beta 3 protein only

Product Datasheets

QK054

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Qk054

Formulation	Lyophilized from acetonitrile/TFA
Reconstitution	Resuspend in 10 mM HCl at >100 µg/mL, prepare single use aliquots, add carrier protein if desired.
Shipping	The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below.
Stability & Storage:	Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Scientific Data

Bioactivity

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TGF-beta 3 activity is determined using a TGF-beta 3-responsive firefly luciferase reporter in HEK293T cells. Cells are treated in triplicate with a serial dilution of TGF-beta 3 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC₅₀ = 50 pg/ml (1.97 pM).

SDS-PAGE

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TGF beta 3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.

Reconstitution Calculator

Background: TGF-beta 3

TGF-beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF- beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-like convertase to generate a 220 aa latency-associated peptide (LAP) and a 112 aa mature TGF-beta 3 (10, 11). Mature human TGF- beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF- beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases (12, 13). It can also be activated by extreme pH and reactive oxygen species (1-5, 12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF- beta RI or ALK-1, to modulate transcription through Smad phosphorylation (14-16). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF- beta RIII and endoglin) and the subsequent initiation of Smad-dependent or -independent signaling pathways (15, 17, 18).

References

Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
Doetschman, T. et al. (2012) Genesis 50:59.
Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
Chang, H. et al. (2002) Endocr. Rev. 23:787.
Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
Derynck, R. et al. (1988) EMBO J. 7:3737.
Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
Munger, J.S. et al. (1997) Kidney Int 51:1376.
Wipff, P.J. and B. Hinz (2008) Eur J Cell Biol 87:601.
Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

Long Name

Transforming Growth Factor beta 3

Entrez Gene IDs

7043 (Human); 21809 (Mouse); 25717 (Rat)

Alternate Names

ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

Product Specific Notices

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.