Home / Sonic Hedgehog/Shh / Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Catalog #: 1845-SH
61 Citations 5 Reviews Datasheet / COA / SDS
GMP Version Available: 1845-GMP
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Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
1845-SH-025/CF
1845-SH-100/CF

With Carrier

Catalog # Availability Size / Price Qty
1845-SH-025
1845-SH-100
1845-SH-500
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Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus Protein SEC-MALS.
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Product Details
Citations (61)
FAQs
Reviews (5)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
Source
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
Accession #
NP_000184.1
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
20 kDa
SDS-PAGE
22 kDa, reducing conditions

Product Datasheets

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1845-SH (with carrier)

Product Datasheet
COA
SDS

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1845-SH/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1845-SH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1845-SH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human SHH Protein (Catalog # 1845-SH) has a molecular weight (MW) of 21.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Bioactivity Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Bioactivity View Larger

Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1-0.4 μg/mL.

SDS-PAGE Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus SDS-PAGE View Larger

1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.

Mass Spectrometry Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Mass Spectrometry View Larger

MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

References
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Citations for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

61 Citations: Showing 1 - 10
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  1. Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency
    Authors: Papandreou, A;Singh, N;Gianfrancesco, L;Budinger, D;Barwick, K;Agrotis, A;Luft, C;Shao, Y;Lenaerts, AS;Gregory, A;Jeong, SY;Hogarth, P;Hayflick, S;Barral, S;Kriston-Vizi, J;Gissen, P;Kurian, MA;Ketteler, R;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Hedgehog target genes regulate lipid metabolism to drive basal cell carcinoma and medulloblastoma
    Authors: Daggubati, V;Vykunta, A;Choudhury, A;Qadeer, Z;Mirchia, K;Saulnier, O;Zakimi, N;Hines, K;Paul, M;Wang, L;Jura, N;Xu, L;Reiter, J;Taylor, M;Weiss, W;Raleigh, D;
    Research square
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model
    Authors: Ito, R;Kimura, A;Hirose, Y;Hatano, Y;Mima, A;Mae, SI;Keidai, Y;Nakamura, T;Fujikura, J;Nishi, Y;Ohta, A;Toyoda, T;Inagaki, N;Osafune, K;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Production of parathyroid-like cells from thyroid stem cells in co-culture environment
    Authors: Ö Karabiyik, GD Nozhatzade, A Tuncer, G Torun Köse, E Hacihasano, F Sahin, E Aysan
    Medicine, 2022-12-02;101(48):e32009.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  5. Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis
    Authors: X Li, PJ Gordon, JA Gaynes, AW Fuller, R Ringuette, CP Santiago, V Wallace, S Blackshaw, P Li, EM Levine
    Elife, 2022-12-02;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Inferring and perturbing cell fate regulomes in human brain organoids
    Authors: JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein
    Nature, 2022-10-05;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  7. Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells
    Authors: DS Lee, YJ Song, HR Gug, JH Lee, HS Bae, JC Park
    Stem Cells International, 2022-09-27;2022(0):1092184.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Parkinson's Disease-Specific Autoantibodies against the Neuroprotective Co-Chaperone STIP1
    Authors: JSY Tan, B Lee, J Lim, DR Ma, JX Goh, SY Goh, MY Gulam, SM Koh, WW Lee, L Feng, Q Wang, Y Chao, O Rötzschke, EK Tan
    Cells, 2022-05-16;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. CNPY4 inhibits the Hedgehog pathway by modulating membrane sterol lipids
    Authors: M Lo, A Sharir, MD Paul, H Torosyan, C Agnew, A Li, C Neben, P Marangoni, L Xu, DR Raleigh, N Jura, OD Klein
    Nature Communications, 2022-05-03;13(1):2407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes
    Authors: A Moore, K Chinnaiya, DW Kim, S Brown, I Stewart, S Robins, GKC Dowsett, C Muir, M Travaglio, JE Lewis, F Ebling, S Blackshaw, A Furley, M Placzek
    Frontiers in Neuroscience, 2022-04-07;16(0):832961.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model
    Authors: L Torz, K Niss, S Lundh, JC Rekling, CD Quintana, SED Frazier, AJ Mercer, A Cornea, CV Bertelsen, MK Gerstenber, AMK Hansen, M Guldbrandt, J Lykkesfeld, LM John, JC Villaescus, N Petersen
    International Journal of Molecular Sciences, 2022-03-17;23(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Generation and characterization of motor neuron progenitors and motor neurons using metachromatic leukodystrophy-induced pluripotent stem cells
    Authors: MA Hossain, M Hasegawa-O, Y Manome, M Igarashi, C Wu, K Suzuki, J Igarashi, T Iwamoto, HJ Okano, Y Eto
    Oncogene, 2022-02-21;31(0):100852.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Suspension culture promotes serosal mesothelial development in human intestinal organoids
    Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
    Cell Reports, 2022-02-15;38(7):110379.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  14. Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog
    Authors: K Kerekes, M Trexler, L Bányai, L Patthy
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Protein
    Applications: Pull-Down Assay
  15. Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons
    Authors: AM Earley, LF Burbulla, D Krainc, R Awatramani
    Scientific Reports, 2021-11-15;11(1):22257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
    Authors: A Azatyan, S Zhang, A Darabi, P Siesjö, T Wang, PG Zaphiropou
    Cancers, 2021-10-13;13(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  17. In vitro monitoring of HTR2A-positive neurons derived from human-induced pluripotent stem cells
    Authors: K Nakai, T Shiga, R Yasuhara, AK Sarkar, Y Abe, S Nakamura, Y Hoashi, K Kotani, S Tatsumoto, H Ishikawa, Y Go, T Inoue, K Mishima, W Akamatsu, K Baba
    Scientific Reports, 2021-07-29;11(1):15437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Resolving organoid brain region identities by mapping single-cell genomic data to reference atlases
    Authors: JS Fleck, F Sanchís-Ca, Z He, M Santel, MJ Boyle, JG Camp, B Treutlein
    Cell Stem Cell, 2021-03-11;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  19. Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss
    Authors: L Cui, J Zheng, Q Zhao, JR Chen, H Liu, G Peng, Y Wu, C Chen, Q He, H Shi, S Yin, RA Friedman, Y Chen, MX Guan
    JCI Insight, 2020-12-03;5(23):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson's disease
    Authors: JL Goggi, L Qiu, MC Liao, S Khanapur, L Jiang, R Boominatha, SV Hartimath, P Cheng, FF Yong, V Soh, X Deng, YM Lin, A Haslop, PW Tan, X Zeng, JWL Lee, Z Zhang, P Sadasivam, EK Tan, SK Luthra, WD Shingleton, SKW Oh, L Zeng, EG Robins
    Stem Cell Res Ther, 2020-08-08;11(1):347.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Trp53 and Rb1 regulate autophagy and ligand-dependent Hedgehog signaling
    Authors: CR Cochrane, V Vaghjiani, A Szczepny, WSN Jayasekara, A Gonzalez-R, K Kikuchi, GW McCaughan, A Burgess, DJ Gough, DN Watkins, JE Cain
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Genetic landscape and ligand-dependent activation of sonic hedgehog-Gli1 signaling in chordomas: a novel therapeutic target
    Authors: C Yang, L Yong, C Liang, Y Li, Y Ma, F Wei, L Jiang, H Zhou, G He, X Pan, B Hai, J Wu, Y Xu, Z Liu, X Liu
    Oncogene, 2020-05-14;0(0):.
    Species: Human
    Sample Types:
    Applications: Cell Culture
  23. Insulin Resistance Promotes Parkinson's Disease through Aberrant Expression of &alpha-Synuclein, Mitochondrial Dysfunction, and Deregulation of the Polo-Like Kinase 2 Signaling
    Authors: CT Hong, KY Chen, W Wang, JY Chiu, D Wu, TY Chao, CJ Hu, KD Chau, OA Bamodu
    Cells, 2020-03-17;9(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  24. Cardioprotective effect of sonic hedgehog ligand in pig models of ischemia reperfusion
    Authors: B Ghaleh, J Thireau, O Cazorla, R Soleti, V Scheuerman, A Bizé, L Sambin, F Roubille, R Andriantsi, MC Martinez, A Lacampagne
    Theranostics, 2020-03-04;10(9):4006-4016.
    Species: Porcine
    Sample Types: In Vivo
    Applications: In Vivo
  25. Generation of Otic Lineages from Integration-Free Human-Induced Pluripotent Stem Cells Reprogrammed by mRNAs
    Authors: SL Boddy, R Romero-Gue, AR Ji, C Unger, L Corns, W Marcotti, MN Rivolta
    Stem Cells Int, 2020-03-01;2020(0):3692937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies
    Authors: WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa
    Cell Rep, 2019-12-10;29(11):3405-3420.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance
    Authors: J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron
    Cell Rep, 2019-12-10;29(11):3356-3366.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. A Sexually Dimorphic Role for STAT3 in Sonic Hedgehog Medulloblastoma
    Authors: CL White, WSN Jayasekara, D Picard, J Chen, DN Watkins, JE Cain, M Remke, DJ Gough
    Cancers (Basel), 2019-11-01;11(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  29. The p75NTR influences cerebellar circuit development and adult behavior via regulation of cell cycle duration of granule cell progenitors
    Authors: JP Zanin, JL Verpeut, Y Li, MW Shiflett, SS Wang, V Santhakuma, WJ Friedman
    J. Neurosci., 2019-10-03;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation
    Authors: S Park, JY Kim, S Myung, N Jung, Y Choi, SC Jung
    Int J Mol Sci, 2019-06-01;20(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. WNT/NOTCH Pathway Is Essential for the Maintenance and Expansion of Human MGE Progenitors
    Authors: L Ma, Y Wang, Y Hui, Y Du, Z Chen, H Feng, S Zhang, N Li, J Song, Y Fang, X Xu, L Shi, B Zhang, J Cheng, S Zhou, L Liu, X Zhang
    Stem Cell Reports, 2019-05-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Influence of SHH/GLI1 axis on EMT mediated migration and invasion of breast cancer cells
    Authors: SK Riaz, Y Ke, F Wang, MA Kayani, MFA Malik
    Sci Rep, 2019-04-29;9(1):6620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects
    Authors: J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi
    Cell Rep, 2019-02-26;26(9):2494-2508.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients
    Authors: M Elkouris, G Kouroupi, A Vourvoukel, N Papagianna, V Kaltezioti, R Matsas, L Stefanis, M Xilouri, PK Politis
    Front Cell Neurosci, 2019-02-22;13(0):58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes
    Authors: C Lang, KR Campbell, BJ Ryan, P Carling, M Attar, J Vowles, OV Perestenko, R Bowden, F Baig, M Kasten, MT Hu, SA Cowley, C Webber, R Wade-Marti
    Cell Stem Cell, 2018-11-29;0(0):.
  36. Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells
    Authors: KKB Tan, WWM Lim, C Chai, M Kukumberg, KL Lim, ELK Goh, EKF Yim
    Sci Rep, 2018-06-22;8(1):9567.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia
    Authors: C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu
    Mucosal Immunol, 2018-06-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Efficient induction of functional ameloblasts from human keratinocyte stem cells
    Authors: X Hu, JW Lee, X Zheng, J Zhang, X Lin, Y Song, B Wang, X Hu, HH Chang, Y Chen, CP Lin, Y Zhang
    Stem Cell Res Ther, 2018-05-02;9(1):126.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018-04-13;50(4):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells
    Authors: G Shall, M Menosky, S Decker, P Nethala, R Welchko, X Leveque, M Lu, M Sandstrom, U Hochgeschw, J Rossignol, G Dunbar
    Int J Mol Sci, 2018-03-02;19(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  41. PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway
    Authors: X Ye, W Hong, B Hao, G Peng, L Huang, Z Zhao, Y Zhou, M Zheng, C Li, C Liang, E Yi, J Pu, B Li, P Ran
    Respir. Res., 2018-03-02;19(1):37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Sonic hedgehog signaling pathway promotes INSM1 transcription factor in neuroendocrine lung cancer
    Authors: C Chen, MB Breslin, MS Lan
    Cell. Signal., 2018-03-01;46(0):83-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Coordinated d-cyclin/Foxd1 activation drives mitogenic activity of Sonic Hedgehog signaling pathway
    Authors: DM Fink, MR Sun, GW Heyne, JL Everson, HM Chung, S Park, MD Sheets, RJ Lipinski
    Cell. Signal., 2017-12-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer
    Authors: SY Xie, G Li, C Han, YY Yu, N Li
    Onco Targets Ther, 2017-11-23;10(0):5605-5619.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. The Golgi matrix protein giantin is required for normal cilia function in zebrafish
    Authors: DJM Bergen, NL Stevenson, REH Skinner, DJ Stephens, CL Hammond
    Biol Open, 2017-08-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. GLI1 blockade potentiates the antitumor activity of PI3K antagonists in lung squamous cell carcinoma
    Authors: S Kasiri, C Shao, B Chen, AN Wilson, P Yenerall, BC Timmons, L Girard, H Tian, C Behrens, II Wistuba, AF Gazdar, J Kim
    Cancer Res., 2017-06-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications
    Authors: L J Spicer
    Domest. Anim. Endocrinol, 2016-12-11;59(0):90-99.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Developmental Reprogramming in Mesenchymal Stromal Cells of Human Subjects with Idiopathic Pulmonary Fibrosis
    Sci Rep, 2016-11-21;6(0):37445.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Anti-proliferation of breast cancer cells with itraconazole: Hedgehog pathway inhibition induces apoptosis and autophagic cell death
    Authors: Hai Zhang
    Cancer Lett., 2016-10-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Hedgehog Acyltransferase as a target in estrogen receptor positive, HER2 amplified, and tamoxifen resistant breast cancer cells.
    Authors: Matevossian A, Resh M
    Mol Cancer, 2015-04-01;14(0):72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
    Authors: Belgacem Y, Borodinsky L
    Proc Natl Acad Sci U S A, 2015-03-17;112(13):4140-5.
    Species: Xenopus
    Sample Types: Embryo
    Applications: Bioassay
  52. Hedgehog signaling stimulates the conversion of cholesterol to steroids.
    Authors: Tang C, Pan Y, Luo H, Xiong W, Zhu H, Ruan H, Wang J, Zou C, Tang L, Iguchi T, Long F, Wu X
    Cell Signal, 2015-01-10;27(3):487-97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Physiological characterisation of human iPS-derived dopaminergic neurons.
    Authors: Hartfield, Elizabet, Yamasaki-Mann, Michiko, Ribeiro Fernandes, Hugo J, Vowles, Jane, James, William, Cowley, Sally A, Wade-Martins, Richard
    PLoS ONE, 2014-02-21;9(2):e87388.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.
    Authors: Williams E, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev G, Mok J, Lin B, Lu J, Chiao C, Cherney R, Li H, Zhang S, Chang Q
    Hum Mol Genet, 2014-01-12;23(11):2968-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Chondrocyte proliferation regulated by secreted luminal domain of ER stress transducer BBF2H7/CREB3L2.
    Authors: Saito A, Kanemoto S, Zhang Y, Asada R, Hino K, Imaizumi K
    Mol Cell, 2013-12-12;53(1):127-39.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  56. Better prognosis of patients with glioma expressing FGF2-dependent PDGFRA irrespective of morphological diagnosis.
    Authors: Chen, Dongfeng, Persson, Annette, Sun, Yingyu, Salford, Leif G, Nord, David Gi, Englund, Elisabet, Jiang, Tao, Fan, Xiaolong
    PLoS ONE, 2013-04-22;8(4):e61556.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
    Authors: Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S
    Nat Biotechnol, 2013-04-21;31(5):440-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. BMP and TGF-beta pathway mediators are critical upstream regulators of Wnt signaling during midbrain dopamine differentiation in human pluripotent stem cells.
    Authors: Cai J, Schleidt S, Pelta-Heller J, Hutchings D, Cannarsa G, Iacovitti L
    Dev Biol, 2013-01-23;376(1):62-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model.
    Authors: Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S
    PLoS ONE, 2011-06-15;6(6):e19904.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.
    Authors: Fabre PJ, Shimogori T, Charron F
    J. Neurosci., 2010-01-06;30(0):266.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  61. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
    Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
    J. Cell Biol., 2008-03-10;180(5):897-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Recombinant Human Sonic Hedgehog Catalog # 1845-SH and Catalog # 1314-SH?

    • Recombinant Human Sonic Hedgehog, Catalog # 1845-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys24-Gly197 (Cys24Ile-Ile), accession number NP_000184. The Cys24Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 1314-SH does not possess this mutation.

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Reviews for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

Average Rating: 4.8 (Based on 5 Reviews)

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Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
By Anonymous on 04/10/2019
Application: Stem/Immune cell maintenance or differentiation
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
By Anonymous on 11/02/2017
Application: CellProlif
Reason for Rating: Works consistently and low doses

Granule cells of cerebellum culture for 24 hs in the presence of 100 ng/ml of Shh.

Cell Proliferation Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus 1845-SH
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
By Anonymous on 05/13/2016
Application: In vivo study
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
By Juan Zanin on 05/12/2016
Application: CellProlif
Reason for Rating: Cerebellar granule cells respond well on EdU incorporation assay
Cell Proliferation Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus 1845-SH
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
By Anonymous on 02/02/2016
Application: Binding assay/Protein-protein interaction