Recombinant Human Siglec-9 Fc Chimera Protein, CF
Recombinant Human Siglec-9 Fc Chimera Protein, CF Summary
Product Specifications
Human Siglec-9 (Gln18-Gly348) Accession # Q9Y336 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1139-SL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Siglec-9
Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.
The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.
- Crocker, P.R. et al. (1998) Glycobiology 8:v.
- Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
- Crocker, P.R. and A. Varki (2001) Immunology 103:137.
- Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
- Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
- Angata, T. et al. (2000) J. Biol. Chem. 275:22127.
Citations for Recombinant Human Siglec-9 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Sialic Acids on Tumor Cells Modulate IgA Therapy by Neutrophils via Inhibitory Receptors Siglec-7 and Siglec-9
Authors: Chan, C;Lustig, M;Jansen, JHM;Garcia Villagrasa, L;Raymakers, L;Daamen, LA;Valerius, T;van Tetering, G;Leusen, JHW;
Cancers
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeting stromal cell sialylation reverses T cell-mediated immunosuppression in the tumor microenvironment
Authors: Egan, H;Treacy, O;Lynch, K;Leonard, NA;O'Malley, G;Reidy, E;O'Neill, A;Corry, SM;De Veirman, K;Vanderkerken, K;Egan, LJ;Ritter, T;Hogan, AM;Redmond, K;Peng, L;Che, J;Gatlin, W;Jayaraman, P;Sheehan, M;Canney, A;Hynes, SO;Kerr, EM;Dunne, PD;O'Dwyer, ME;Ryan, AE;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Siglec-9 defines and restrains a natural killer subpopulation highly cytotoxic to HIV-infected cells
Authors: OS Adeniji, L Kuri-Cerva, C Yu, Z Xu, M Ho, GM Chew, C Shikuma, C Tomescu, AF George, NR Roan, LC Ndhlovu, Q Liu, K Muthumani, DB Weiner, MR Betts, H Xiao, M Abdel-Mohs
PloS Pathogens, 2021-11-11;17(11):e1010034.
Species: Human, Mouse
Sample Types: In Vivo, Recombinant Proteins
Applications: ELISA Control, In Vivo, Western Blot Control -
Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019-07-25;14(7):e0219480.
Species: Human
Sample Types: Natural Protein
Applications: Bioassay -
Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment
Authors: Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten
Cancer Immunol Res, 2019-04-15;0(0):.
Species: Human
Sample Types: Whole Tissue
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Increased expression of Siglec-9 in chronic obstructive pulmonary disease
Authors: Z Zeng, M Li, M Wang, X Wu, Q Li, Q Ning, J Zhao, Y Xu, J Xie
Sci Rep, 2017-08-31;7(1):10116.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Siglec-9 is upregulated in rheumatoid arthritis and suppresses collagen-induced arthritis through reciprocal regulation of Th17/Treg cell differentiation
Authors: X Wang, D Liu, Y Ning, J Liu, X Wang, R Tu, H Shen, Q Chen, Y Xiong
Scand. J. Immunol, 2017-06-01;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
Sci Rep, 2017-03-08;7(0):44043.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Soluble Siglec-9 suppresses arthritis in a collagen-induced arthritis mouse model and inhibits M1 activation of RAW264.7 macrophages
Authors: Takuya Matsumoto
Arthritis Res Ther, 2016-06-07;18(1):133.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
J Neurosci, 2015-02-11;35(6):2452-64.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Elife, 2014-09-03;3(0):e04066.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Authors: Zou Z, Chastain A, Moir S
PLoS ONE, 2011-09-08;6(9):e24559.
Species: Virus
Sample Types: Virus
Applications: Surface Plasmon Resonance
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