Recombinant Human Siglec-15 His-tag Protein, CF

Catalog #: 10698-SL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10698-SL-050

Recombinant Human Siglec-15 His-tag Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Siglec-15 His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.2-2 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein
Phe20-Tyr251, with a C-terminal 6-His tag

Accession #

Q6ZMC9.1

N-terminal Sequence
Analysis

Phe20

Predicted Molecular Mass

26 kDa

SDS-PAGE

27-37 kDa, under reducing conditions

Product Datasheets

10698-SL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10698-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect is 0.2‑2 μg/mL

Reconstitution Calculator

Background: Siglec-15

Sialic acid-binding Ig-like lectin 15 (Siglec-15) is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Siglecs can be divided into 2 classes: evolutionarily conserved Siglecs and CD33-related Siglecs (2). Siglec-15 belongs to the evolutionarily conserved class and is the only Siglec highly conserved throughout vertebrate evolution (3). Mature human Siglec-15 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% and 84% amino acid sequence identity with mouse and rat Siglec‑15, respectively. Alternative splicing of Siglec-15 generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 is expressed on osteoclasts, macrophages, and dendritic cells (3-7) and binds to the sialyl-Tn antigen (3, 4, 7). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (5-7) and for the production of TGF-beta by tumor-associated macrophages (4). Additionally, Siglec-15 associates with the signaling molecules DAP12 and DAP10 through a lysine residue located in the transmembrane domain (3-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (8). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (8).

References

Humphrey, M.B. and M.C. Nakamura (2016) Clin. Rev. Allergy Immunol. 51:48.
Varki, A. and Angata, T. (2006) Glycobiology, 16:1R.
Angata, T. et al. (2007) Glycobiology 17:838.
Takamiya, R. et al. (2013) Glycobiology 23:178.
Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Wang, J. et al. (2019) Nat. med. 25:656.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Alternate Names

CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15