Recombinant Human sFRP-3 Protein

Name: Recombinant Human sFRP-3 Protein
Brand: R&D Systems
SKU: 7584-SF
Rating: 5 (1 reviews)

Catalog #: 7584-SF
1 Citation 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
7584-SF-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7584-SF-025

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Citations (1)

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human sFRP-3 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED₅₀ for this effect is 0.05-0.25 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human sFRP-3 protein
Ala33-Asn325

Accession #

AAB51298

N-terminal Sequence
Analysis

Ala33

Predicted Molecular Mass

33.2 kDa

SDS-PAGE

39 kDa, reducing conditions

Product Datasheets

7584-SF (with carrier)

Product Datasheet

COA

SDS

7584-SF/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7584-SF

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7584-SF/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: sFRP-3

Secreted Frizzled Related Protein‑3 (sFRP‑3), also known as FRZB, belongs to a family of Wnt-binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1‑4). Mature human sFRP‑3 shares 93% aa sequence identity with mouse and rat sFRP‑3. It neutralizes the bioactivity of Wnt‑1, ‑5a, ‑9a, Xenopus Xwnt‑8, and EGF (5‑8). sFRP‑3 regulates somitic myogenesis and osteoblast differentiation as well as morphogenesis of the cochlear epithelium, hair follicle, and cardiac atrioventricular cushion (6, 8‑10). sFRP‑3 also functions as a tumor suppressor in a variety of cancers including melanoma, medulloblastoma, soft tissue sarcoma, and androgen‑independent prostate cancer. It inhibits epithelial-mesenchymal transition, tumor growth and invasion, and the activity of MMP‑2 and MMP‑9 (7, 11‑13). In contrast, it can promote tumor growth and angiogenesis in renal cancer (14).

References

Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
van Amerongen, R. and R. Nusse (2009) Development 136:3205.
Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
Hoang, B. et al. (1996) J. Biol. Chem. 271:26131.
Wang, S. et al. (1997) Cell 88:757.
Person, A.D. et al. (2005) Dev. Biol. 278:35.
Ekstrom, E.J. et al. (2011) PLoS ONE 6:e18674.
Scardigli, R. et al. (2008) PLoS ONE 3:e2471.
Qian, D. et al. (2007) Dev. Biol. 306:121.
Chung, Y.-S. et al. (2004) J. Bone Miner. Res. 19:1395.
Kongkham, P.N. et al. (2010) Oncogene 29:3017.
Guo, Y. et al. (2008) Cancer Res. 68:3350.
Zi, X. et al. (2005) Cancer Res. 65:9762.
Hirata, H. et al. (2010) Cancer Res. 70:1896.

Long Name

Secreted Frizzled Related Protein 3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Alternate Names

FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related

Related Research Areas

Citation for Recombinant Human sFRP-3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Wnt Signaling Mediates LTP-Dependent Spine Plasticity and AMPAR Localization through Frizzled-7 Receptors
Authors: F McLeod, A Bossio, A Marzo, L Ciani, S Sibilla, S Hannan, GA Wilson, E Palomer, TG Smart, A Gibb, PC Salinas
Cell Rep, 2018-04-24;23(4):1060-1071.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay