Recombinant Human SCG3 His-tag Protein, CF Summary
Product Specifications
Phe20-Leu468, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10374-SC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human SCG3 His-tag (Catalog # 10374-SC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
Reconstitution Calculator
Background: SCG3
Secretogranin III, also known as SCG3, is a member of the chromogranin-secretogranin family of neuroendocrine secretory proteins, collectively referred to as granins (1). Additional members of this family include secretogranin II, IV, and V, chromogranin A and B, and a few additional related proteins (1, 2). SCG3 is synthesized as an N-glycosylated protein and two isoforms can be generated from alternative splicing (2). Mature SCG3 contains three functional regions: an N‑terminal cholesterol-binding domain, a chromogranin A binding domain and a C-terminal carboxypeptidase E binding domain (3). Human SCG3 shares 88% and 87% amino acid sequence identity with mouse and rat SCG3, respectively. Granins are highly acidic proteins that are a major component of secretory granules of endocrine, neuroendocrine, and neuronal cells (4-6). Calcium binding at low pH and intermolecular aggregation plays a role in granins' sorting of secretory proteins at the trans-Golgi network (3-6). Within the trans-Golgi network, SCG3 binds to chromogranin A (CgA), membrane-associated carboxypeptidase E (CPE), and cholesterol‑rich membrane domain (3-5). SCG3 has been implicated in diabetic retinopathy (DR) and retinopathy of prematurity (ROP) as a disease-associated endothelial ligand, thus suggesting SCG3 is a promising target for novel antiangiogenic therapy of DR and ROP (7, 8).
- Peinado, J. R. et al. (2006) Endocrinology. 147:1408.
- Bartolomucci, A. et al. (2011) Endocr. Rev. 32:755.
- Hosaka M. et al. (2010) Endocr. J. 57:275.
- Sun, M. et al. (2013) Traffic. 14:205.
- Hosaka, M. et al. (2004) J. Biol. Chem. 279:3627.
- Hosaka, M. et al. (2005) J. Cell Sci. 118:4785.
- LeBlanc, M. E. et al. (2017) J. Exp. Med. 214:1029.
- Tang, F. et al. (2017) Biochem. Biophys. Res. Commun. 495:781.
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