Recombinant Human QBRICK Fc Chimera Protein, CF

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5100-QB-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human QBRICK Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. Kiyozumi, D. et al. (2005) Exp. Cell Res. 306:9. When 5 x 104 cells/well are added to rhQBRICK/Fc Chimera coated plates (2.5 µg/mL, 100 µL/well), approximately 60%‑80% will adhere after 1 hour at 37° C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human QBRICK/FREM1 protein
Human QBRICK
(Ser22 - Glu384)
Accession # Q5H8C1
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser22
Structure / Form
Oligomer
Predicted Molecular Mass
67.8 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions

Product Datasheets

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5100-QB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5100-QB

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: QBRICK/FREM1

QBRICK, also known as Frem1 (Fras1-related extracellular matrix gene1) is a 244 kDa (predicted), secreted, extracellular matrix glycoprotein and member of the Fras1 family of proteins (1 - 3). Human QBRICK is synthesized as a 2179 amino acid (aa) precursor that has a 21 aa signal sequence and a 2158 aa mature chain (SwissProt #: Q5H8C1). The mature chain is made up of an N-terminal variable region domain containing an Arg-Gly-Asp (RGD) cell adhesive motif, 12 consecutive chondroitin sulfate proteoglycan (CSPG) repeats of approximately 120 aa, a Calx-beta domain, a second RGD sequence, and a C-terminal C-type lectin-like domain, respectively (1). In addition, there are five potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for human QBRICK. Because of the characteristic feature of the 12 CSPG repeats, the protein was named QBRICK: “Q” stands for queen and is taken from the queen being the twelfth in a suit of playing cards, and “BRICK” stands for the repeating unit (1). Human QBRICK shares 78% aa sequence identity with mouse QBRICK. QBRICK is localized to the basement membrane in mesenchymal tissue (3). QBRICK plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. QBRICK mediates cell-substratum adhesion through alpha V or alpha 8 integrins (1 - 4). Mutations in QBRICK and other Fras1 family proteins (i.e. Fras1 and Frem2) are associated with Fraser syndrome, a recessive multiorgan disorder characterized by crypthophthalmos, syndactyly, renal agenesis, and a variety of morphogenetic defects (2 - 4). It is postulated that QBRICK, Fras1 and Frem2 make up a ternary complex that act together to ensure adhesion between the epidermal basement membrane and the underlying mesenchyme in embryonic skin (3).

References
  1. Kiyozumi, D. et al. (2005) Exp. Cell Res. 306:9.
  2. Smyth, I. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:13560.
  3. Kiyozumi, D. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:11981.
  4. Smyth, I. and P. Scambler (2005) Hum. Mol. Genet. 14:R269.
Long Name
FRAS-1 Related Extracellular Matrix Protein 1
Entrez Gene IDs
158326 (Human)
Alternate Names
BNAR; C9orf143chromosome 9 open reading frame 154; C9orf145C9orf154extracellular matrix protein QBRICK; C9orf154; DKFZp686M16108; FLJ25461; FRAS1 related extracellular matrix 1; FRAS1-related extracellular matrix protein 1; FREM1; Protein QBRICK; QBRICK; TILRR

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