Recombinant Human PTGES2 Protein, CF

Catalog #: 7627-PS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7627-PS-020

R&D Systems Recombinant Proteins and Enzymes

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All Prostaglandin E Synthase 2/PTGES2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PTGES2 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Source

E. coli-derived human Prostaglandin E Synthase 2/PTGES2 protein
Glu88-His377, with an N-terminal Met and 6-His tag

Accession #

Q9H7Z7

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

28-32 kDa, reducing conditions

Product Datasheets

7627-PS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7627-PS

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Prostaglandin E Synthase 2/PTGES2

PTGES2, also known as membrane-associated prostaglandin E synthase (mPGES) catalyzes the conversion of prostaglandin H₂ (PGH₂) to prostaglandin E₂ (PGE₂). Its N-terminal domain anchors it in the Golgi membrane, and its catalytic domain resides in the cytoplasm. Proteolysis within the cell generates a truncated soluble form beginning with amino acid 88 that resides in the cytosol (1). PTGES2 production of PGE₂ is coupled to both COX-1 and COX-2 (2). PTGES2 is expressed in many tissues, with highest expression in brain, heart, skeletal muscle, kidney, and liver (1). In addition to its PGE₂ production, PTGES2 activates transcription regulated by a gamma-interferon-activated transcription element (3). The protein may also play a role in colorectal cancer progression (4).

References

Tanikawa, N. et al. (2002) Biochem. Biophys. Res. Commun. 291:884.
Murakami, M. et al. (2003) J. Biol. Chem. 278:37937.
Hu, J. et al. (2002) J. Biol. Chem. 277:30253.
Seo, T. et al. (2009) Virchows Arch. 454:667.

Entrez Gene IDs

80142 (Human); 96979 (Mouse); 311865 (Rat)

Alternate Names

C9orf15; chromosome 9 open reading frame 15; EC 5.3.99.3; FLJ14038; gamma-interferon-activated transcriptional element-binding factor 1; GATE-binding factor 1; GBF1; membrane-associated prostaglandin E synthase 2; Microsomal prostaglandin E synthase 2; microsomal prostaglandin E synthase-2; mPGES2; mPGES-2; PGES2; PGES2MGC11289; prostaglandin E synthase 2; PTGES2