Recombinant Human Plexin B1 (Sema domain) His Protein, CF
Recombinant Human Plexin B1 (Sema domain) His Protein, CF Summary
Product Specifications
Leu20-Gln535, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10803-PB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1.00 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human Plexin B1 His-tag (Catalog # 10803-PB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.
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When Recombinant Human Plexin B1 (Sema domain) His-tag (Catalog # 10803-PB) is coated at 1.00 µg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera [[catalogNumber:(7470-S4)]] binds with an ED50 of 80.0-480 ng/mL.
Reconstitution Calculator
Background: Plexin B1
Plexin B1, also known as SEP, is a type I transmembrane glycoprotein that belongs to the Plexin family of semaphorin receptors. In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (1). Plexins share a common structural architecture: A large extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and a conserved ~600 amino acid (aa) cytoplasmic region (2, 3). Human Plexin B1 contains one Semaphorin domain, two plexin-semaphorin-integrin (PSI) domains, and three immunoglobin-plexin-transcription factor (IPT) repeats in the ECD. The mature ECD of human Plexin B1 shares 82% aa sequence identity with the ECD of mouse Plexin B1. Like semaphorins, plexins can be soluble or membrane bound and Plexin B1 can be cleaved into an ~200 kDa, soluble alpha -subunit and an ~100 kDa membrane bound beta -subunit (2). Plexin B1 is a receptor for Semaphorin 4D/CD100 and it can form a receptor complex with Neuropilins, MET, and EGF R2 (4). Plexin B1 is expressed in chick retinal ganglion neurons and hippocampal neurons with a peak during the myelination period (5). Plexin B1 also plays a role in GABAergic development, changes of actin cytoskeleton, axon guidance, cell migration, and invasive growth (6, 7).
- Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789
- Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
- Negishi, M. et al. Cell. Mol. Life Sci. (2005) 62:1363.
- Franco, M. and Tamagnone, L. (2008) EMBO Rep. 9:865.
- Moreau-Fauvarque, C. et al. J. Neurrosci (2003) 23:9229.
- Swiercz, J. et al. J Cell Biol (2004) 165(6):869.
- Giordano, S. et al. Nat Cell Biol. (2002) 4(9):720.
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