Recombinant Human Plexin B1 His-tag Protein, CF

Catalog #: 10677-PB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10677-PB-050

Recombinant Human Plexin B1 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Plexin B1 His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B1 His-Tag protein is coated at 1 µg/mL, Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4) binds with an ED₅₀ of 0.02-0.12 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Plexin B1 protein
Leu20-Gln1490 (RRRR1302-1305AAAA), with a C-terminal 6-His tag

Accession #

O43157.3

N-terminal Sequence
Analysis

Leu20

Predicted Molecular Mass

158 kDa

SDS-PAGE

215-250 kDa, under reducing conditions

Product Datasheets

10677-PB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10677-PB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Plexin B1 His-Tag (Catalog # 10677-PB) is coated at 1 µg/mL, Recombinant Human Semaphorin 4D Fc Chimera (7470-S4) binds with an ED₅₀ of 0.02-0.12 μg/mL.

Reconstitution Calculator

Background: Plexin B1

Plexin B1, also known as SEP, is a type I transmembrane glycoprotein that belongs to the Plexin family of semaphorin receptors. In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (1). Plexins share a common structural architecture: A large extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and a conserved ~600 amino acid (aa) cytoplasmic region (2, 3). The ECD of mature, human Plexin B1 contains one Semaphorin domain, two plexin-semaphorin-integrin (PSI) domains, and three immunoglobin-plexin-transcription factor (IPT) repeats and shares 82% amino acid sequence identity with the ECD of mouse Plexin B1. Like semaphorins, plexins can be soluble or membrane bound and Plexin B1 can be cleaved into an ~200 kDa, soluble alpha -subunit and an ~100 kDa membrane bound beta -subunit (2). Plexin B1 is a receptor for Semaphorin 4D/CD100 and it can form a receptor complex with Neuropilins, MET, and EGF R2 (4). Plexin B1 is expressed in chick retinal ganglion neurons and hippocampal neurons with a peak during the myelination period (5). Plexin B1 also plays a role in GABAergic development, changes of actin cytoskeleton, axon guidance, cell migration, and invasive growth (6, 7).

References