Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI1086 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI1086-050

Recombinant Human PD-1 Fc Chimera Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PD-1 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Why choose R&D Systems Avi-tag PD-1 Protein?

Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the His Avi-tag PD-1 protein here His Avi-tag PD-1 Protein

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) that produces 50% of the optimal binding response is 10-90 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human PD-1 protein

Human PD-1
(Leu25-Thr168)
Accession # Q15116.3

IEGRMD

Human IgG₁
(Pro100-Lys330)

HP, 3x (GGGS)

Avi-tag

Accession #

Q15116.3

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

45 kDa

SDS-PAGE

62-88 kDa, under reducing conditions

Product Datasheets

AVI1086

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1086

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) is immobilized at 1 µg/mL, 100 µg/well, Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) binds with an ED₅₀ of 0.01-0.09 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human PD-1 Fc Chimera Avi-tag (Catalog # AVI1086) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-88 kDa and 120-180 kDa, respectively.

Reconstitution Calculator

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

References