Recombinant Human Parkin Protein, CF Summary
Product Specifications
Reaction conditions will need to be optimized for each specific application. As supplied, Parkin has negligible E3 ligase activity as determined by the lack of autoubiquitination in an in vitro assay. Parkin ligase activity is greatly enhanced by phosphorylation.
Met1 - Val465
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-160
| Formulation | Supplied as a solution in Tris, NaCl, Brij-35, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Parkin
The Parkin protein (encoded by the PARK2 gene) is an E3 ubiquitin ligase that plays an essential role in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which the defective Parkin protein contributes to dopaminergic cell death in this disease is an area of intense investigation. Reported substrates of the Parkin protein include BCL2, GPR37, MIRO1, MFN1, MFN2, TOMM20, USP30, and many others. The structure of the Parkin protein (an RBR-class Ubiquitin ligase) has been reported by multiple groups. These reports showed that the ligase is folded upon itself to produce an auto-inhibited state. This auto-inhibition is relieved by interactions with PINK1 kinase, which can phosphorylate both the Parkin protein and Ubiquitin at serine residue number 65, and pS65 phospho-Ubiquitin by mechanisms that are under investigation. In vitro, the Parkin protein may be activated by treatment with recombinant PINK1, or addition of low concentrations of pS65-phospho-Ubiquitin. Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo.
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Bingol, B. et al. (2014) Nature 510: 370.
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Ordureau, A. et al. (2014) Mol. Cell 56: 360.
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Riley, B.E. et al. (2013) Nat. Comm. 4: 1982.
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Saraff, S.A. et al. (2013) Nature 496: 372.
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Spratt, D.E. et al. (2013) Nat. Comm. 4: 1983.
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Trempe, J.F. et al. (2013) Science 340: 1451.
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Wauer T. et. al. (2015) Nature 524: 370.
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Wauer T. & Komander, D. (2013) EMBO J 32: 2099.
Citations for Recombinant Human Parkin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination
Authors: Ardah, MT;Radwan, N;Khan, E;Kitada, T;Haque, ME;
International journal of molecular sciences
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy
Authors: ND Roverato, C Sailer, N Catone, A Aichem, F Stengel, M Groettrup
Cell Reports, 2021-03-16;34(11):108857.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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At what molecular weight does Recombinant Human Parkin Protein (Catalog # E3-160) run in SDS-PAGE?
The theoretical molecular weight for this protein is 52 kDa, however many RING-containing proteins (such as Parkin) run at a lower apparent molecular weight than expected. We have found that this protein runs around 40 kDa.
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