Recombinant Human Pappalysin-1/PAPP-A (aa 82-1627), CF
Recombinant Human Pappalysin-1/PAPP-A (aa 82-1627), CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2487-ZNF
Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2, and CHAPS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
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Background: Pappalysin-1/PAPP-A
Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP‑A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca2+ and are required for the cleavage of IGFBP-4, but not IGFBP-5, by PAPP-A (5). As an active protease, recombinant human (rh) PAPP-A cleaves IGFBP-4 and IGFBP-5. This full-length rhPAPP-A, which contains the five C-terminal Sushi domains, is approximately 9-fold more active for cleavage of IGFBP-5 than the C-terminally truncated rhPAPP-A (Catalog # 2487‑ZN).
- Boldt, H.B. et al. (2001) Biochem. J. 358:359.
- Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
- Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
- Conover, C.A. et al. (2004) Development 131:1187.
- Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.
Citations for Recombinant Human Pappalysin-1/PAPP-A (aa 82-1627), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways
Authors: W Li, H Li, L Zhou, Z Wang, B Hua
Mediators Inflamm., 2019-09-10;2019(0):8436985.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
EWS-FLI-1 creates a cell surface microenvironment conducive to IGF signaling by inducing pappalysin-1
Authors: P Jayabal, PJ Houghton, Y Shiio
Genes Cancer, 2017-11-01;8(11):762-770.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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