Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF
Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF Summary
Product Specifications
Asp21-Gln510, with a C-terminal 6-His tag
Analysis
56 kDa
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10261-OL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-75 kDa on reducing gel.
Reconstitution Calculator
Background: Olfactomedin-4/OLFM4
Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an approximately 64-72 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein (1-3). It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers (4-7), and is up-regulated in epithelial cells during inflammation (8). Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions (2). Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells (9). OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer (10-11).
- Zhang, J. et al. (2002) Gene 283:83.
- Grover, P.K. et al. (2010) Cancer Metastasis Rev. 29:761.
- Guette, C. et al. (2015) Proteomics Clin. Appl. 9:58.
- Zhang, X. et al. (2004) Cancer Res. 64:2474.
- Kobayashi, D. et al. (2007) Cancer Sci. 98:334.
- Koshida, S. et al. (2007) Cancer Sci. 98:315.
- Yu, L. et al. (2011) Int. J. Gynecol. Cancer 21:367.
- Shinozaki, S. et al. (2001) Gut 48:623.
- Liu, W. et al. (2006) Exp. Cell Res. 312:1785.
- Xiong, B. et al. (2017) Biomed. Pharmacother. 86:67.
- Li, H. et al. (2015) Sci. Rep. 5:16974.
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