Recombinant Human MOG Protein, CF

Catalog #: 8535-MO
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8535-MO-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MOG Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human MOG, immobilized at 1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Source

Mouse myeloma cell line, NS0-derived human MOG protein
Gly30-Gly154, with a substitution at Asp131Ala and a C-terminal 10-His tag

Accession #

Q16653

N-terminal Sequence
Analysis

Gly30

Predicted Molecular Mass

16 kDa

SDS-PAGE

20-30 kDa, reducing conditions

Product Datasheets

8535-MO

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8535-MO

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MOG

Myelin oligodendrocyte glycoprotein (MOG) is 28 kDa single-pass transmembrane glycoprotein that is a member of the Ig superfamily (1, 2). Human MOG is synthesized with a 29 amino acid (aa) signal sequence, a 125 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 72 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. The ECD of mature human MOG shares 90% aa sequence identity with the ECD of both mouse and rat MOG. Unlike mouse and rat, human MOG has seven splice variants, including a soluble ECD isoform and multiple isoforms that have a truncated cytoplasmic domain (3, 4). Dimerization of MOG occurs via the extracellular Ig-like domain (5-8). MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane (9). MOG expression in the brain can be used as a temporal biomarker for myelin development. MOG is an important antigenic target for autoimmune diseases that mediate demyelination in the CNS (10). In vivo administration of exogenous MOG protein or peptide induces experimental autoimmune encephalomyelitis (EAE) in multiple animal species (11, 12). EAE is used as an animal model for multiple sclerosis and related CNS demyelinating diseases. MOG is thought to function as an adhesion molecule as well as a mediator of immune activation in the CNS (9, 13).

References

Johns, T.G. and C.C. Bernard (1999) J. Neurochem. 72:1.
Pham-Dinh, D. et al. (1993) Proc. Natl. Acad. Sci. U S A 90:7990.
Hilton, A.A. et al. (1995) J. Neurochem. 65:309.
Slavin, A.J. et al. (1997) Dev. Neurosci. 19:69.
Abo, S. et al. (1993) Biochem. Mol. Biol. Int. 30:945.
Amiguet, P. et al. (1992) J. Neurochem. 58:1676.
Bettadapura, J. et al. (1998) J. Neurochem. 70:1593.
Clements, C.S. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:11059.
Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
von Budingen, H.C. et al. (2004) Eur. J. Immunol. 34:2072.
Rangachari, M. and V.K. Kuchroo (2013) J. Autoimmun. 45:31.
Tompkins, S.M. et al. (2002) J. Immunol. 168:4173.
Garcia-Vallejo, J.J. et al. (2014) J. Exp. Med. 211:1465.

Long Name

Myelin Oligodendrocyte Glycoprotein

Entrez Gene IDs

4340 (Human); 17441 (Mouse)

Alternate Names

MGC26137; MOG Ig-AluB; MOG; MOGIG2; myelin oligodendrocyte glycoprotein; myelin-oligodendrocyte glycoprotein

Related Research Areas

Citation for Recombinant Human MOG Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Remibrutinib (LOU064) inhibits neuroinflammation driven by B cells and myeloid cells in preclinical models of multiple sclerosis
Authors: Nuesslein-Hildesheim, B;Ferrero, E;Schmid, C;Huck, C;Smith, P;Tisserand, S;Rubert, J;Bornancin, F;Eichlisberger, D;Cenni, B;
Journal of neuroinflammation
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay