Recombinant Human MDL-1/CLEC5A Protein, CF Summary
Product Specifications
Tyr26-Lys188, with an N-terminal 9-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8544-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: MDL-1/CLEC5A
MDL-1 (Myeloid DAP12-associating lectin 1), also known as CLEC5A, is an approximately 40 kDa member of the C-type lectin family (1). Mature human MDL-1 is a glycosylated type 2 transmembrane protein that associates into a homodimer on the cell surface (2). It consists of a 161 amino acid (aa) extracellular domain (ECD) with one C-type lectin (CTL) domain and a juxtamembrane stalk region, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (3). Within the ECD, human MDL-1 shares 67% and 69% aa sequence identity with mouse and rat MDL-1, respectively. The transmembrane segment contains a lysine residue that mediates interactions with the signaling protein DAP12 (3). MDL-1 is expressed on monocytes, macrophages, and neutrophils (3, 4). Its expression is up-regulated on monocytes in rheumatoid arthritis (5). MDL-1 functions as a cell attachment receptor for all four serotypes of Denguevirus as well as Japanese encephalitis virus, although the short isoform binds significantly more weakly (2, 6, 7). These interactions trigger DAP12 phosphorylation, the production of multiple inflammatory cytokines, vascular leakage, and disruption of the blood-brain barrier (6-8).
- Hoving, J.C. et al. (2014) Cell. Microbiol. 16:185.
- Watson, A.A. et al. (2011) J. Biol. Chem. 286:24208.
- Bakker, A.B.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9792.
- Aoki, N. et al. (2009) J. Leukoc. Biol. 85:508.
- Chen, D.-Y. et al. (2014) PLoS ONE 9:e86105.
- Chen, S.-T. et al. (2012) PLoS Pathogens 8:e1002655.
- Chen, S.-T. et al. (2008) Nature 453:672.
- Wu, M.-F. et al. (2013) Blood 121:95.
Citation for Recombinant Human MDL-1/CLEC5A Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
CLEC5A mediates Zika virus-induced testicular damage
Authors: HW Wang, HH Li, SC Wu, CK Tang, HY Yu, YC Chang, PS Sung, WL Liu, MP Su, GY Yu, LR Huang, CH Chen, SL Hsieh
Journal of Biomedical Science, 2023-02-17;30(1):12.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human MDL-1/CLEC5A Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human MDL-1/CLEC5A Protein, CF and earn rewards!
Have you used Recombinant Human MDL-1/CLEC5A Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
