Recombinant Human M-CSF Biotinylated Protein, CF

Catalog #: BT216 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
BT216-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human M-CSF Biotinylated Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (Catalog # 329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of &Biotinylated Recombinant Human M‑CSF (Catalog # BT216) that produces 50% of the optimal binding response is 1.50‑15.0 ng/mL.

Source

E. coli-derived human M-CSF protein
Glu33-Ser190, with an N-terminal Met

Accession #

NP_757350.2

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Biotinylated via amines

Predicted Molecular Mass

18.5 kDa

SDS-PAGE

15-22 kDa, under reducing conditions.

Product Datasheets

BT216

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT216

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (329-MR) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human M‑CSF Protein (Catalog # BT216) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-22 kDa and 30-40 kDa, respectively.

Reconstitution Calculator

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode
M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N‑terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M‑CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

References

Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
Ryan, G.R. et al. (2001) Blood 98:74.
Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
Nandi, S. et al. (2006) Blood 107:786.
Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
Koths, K. (1997) Mol. Reprod. Dev. 46:31.
Jang, M-H. et al. (2006) J. Immunol. 177:4055.
Kawasaki, E.S. et al. (1985) Science 230: 291.
Wong, G.G. et al. (1987) Science 235:1504.

Long Name

Macrophage Colony Stimulating Factor

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Alternate Names

colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930