Recombinant Human LYPD8 Fc Chimera Protein, CF

Catalog #: 9087-C4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9087-C4-050

Recombinant Human LYPD8 Fc Chimera Protein Bioactivity

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LYPD8 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LYPD8 Fc Chimera is coated at 2 µg/mL, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with a typical ED₅₀ = 90-540 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human LYPD8 protein

Human LYPD8 (Leu20-Asn215) Accession # Q6UX82	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6UX82

N-terminal Sequence
Analysis

Leu20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48 kDa

SDS-PAGE

85-100 kDa, reducing conditions

Product Datasheets

9087-C4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9087-C4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

When Recombinant Human LYPD8 Fc Chimera is coated at 2 µg/ml, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with a typical ED₅₀ of 90-540 ng/mL.

Reconstitution Calculator

Background: LYPD8

Ly6/PLAUR domain containing 8 (LYPD8), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR) (1). Mature human LYPD8 contains one uPAR/Ly6 domain and a Ser/Thr/Pro-rich (STP) region that may serve as a target for protease mediated shedding as has been shown for the related C4.4A/LYPD3 molecule (2, 3). Mature human LYPD8 shares 40% amino acid sequence identity with mouse and rat LYPD8.

References