Recombinant Human LRP-10 Fc Chimera Protein, CF

Catalog #: 10647-LR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10647-LR-050

Recombinant Human LRP-10 Fc Chimera Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LRP-10 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LRPAP (Catalog # 4296-LR) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) binds with an ED₅₀ of 12-60 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human LRP-10 protein

Human LRP-10 (His17-Pro438) Accession # NP_054764.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_054764.2

N-terminal Sequence
Analysis

His17

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

72 kDa

SDS-PAGE

84-96 kDa, under reducing conditions

Product Datasheets

10647-LR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10647-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-96 kDa and 160-180 kDa, respectively.

Reconstitution Calculator

Background: LRP-10

LRP-10 (Low-density lipoprotein receptor-related protein 10) is a member of the new subfamily of low-density lipoprotein receptor (LDLR) family that includes two other receptors LRP-3 and LRP-12 (1). It is called LRP-9 in the mice. This unique family is characterized by extracellular CUB domains and large cytoplasmic tails containing acidic dileucine (DXXLL) motifs (2). Mature human LRP-10 consists of a 424 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 252 aa cytoplasmic domain. The ECD contains 4 LDLR-A domains and 2 CUB domains. Within the ECD, human LRP-10 shares 90% aa sequence identity with mouse and rat LRP-10. LRP-10 is expressed in various tissues including brain and may be involved in apolipoprotein internalization (3). It is also localized in Trans‑Golgi network (TGN) and endosomes (2,4). LRP-10 is also identified as a novel sorting receptor for Amyloid precursor protein (APP) and a decrease in LRP-10 function contributes to the pathogenesis of Alzheimer's disease (5).

References

Battle, M. A. et al. (2003) Biochemistry. 42:7270.
Boucher, R. et al. (2008) Histochem. Cell. Biol. 130:315.
Sugiyama, T. et al. (2000) Biochemistry. 39:15817.
Doray, B. et al. (2008) Traffic. 9:1551.
Brodeur, J. et al. (2012) Mol. Neurodegener. 7:31.

Long Name

LDL Receptor-related Protein 10

Entrez Gene IDs

26020 (Human); 65107 (Mouse); 305880 (Rat)

Alternate Names

DKFZp564C1940; low density lipoprotein receptor-related protein 10; low-density lipoprotein receptor-related protein 10; LRP-10; LRP9; MGC142274; MGC142276; MGC8675; MST087