Recombinant Human Laminin alpha 4 Protein, CF

Catalog #: 7340-A4
5 Citations Datasheet / COA / SDS

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7340-A4-050

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Citations (5)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Laminin alpha 4 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of HT1080 human fibrosarcoma cells. The ED₅₀ for this effect is 0.7-2.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 4 protein
Gln826-Ala1816, with a C-terminal 6-His tag

Accession #

EAW48267

N-terminal Sequence
Analysis

Gln826 predicted, sequencing might be blocked, no result obtained

Structure / Form

Monomer

Predicted Molecular Mass

111 kDa

SDS-PAGE

110-120 kDa, reducing conditions

Product Datasheets

7340-A4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7340-A4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Laminin alpha 4

The alpha 4 chain of laminin (LAMA4) is a secreted ~210 kDa glycoprotein that contributes to the formation of LN411 (laminin-8, subunits alpha 4 beta 1 gamma 1), LN421 (laminin-9, subunits alpha 4 beta 2 gamma 1), and LN423 (laminin-14, subunits alpha 4 beta 2 gamma 3) (1-4). Laminin alpha, beta and gamma subunits interact via alpha -helical domains to create cruciform, disulfide-linked, coiled-coil laminin heterotrimers (1, 2). LAMA4 is mainly expressed in mesodermal tissues, and is produced by endothelium, cardiac and skeletal muscle fibers, lung mesenchymal cells, pre-adipocytes, kidney mesangial cells, peripheral nerves and neutrophils (3-8). It is found in the subendothelium in muscle, brain, and bone marrow, and in heart, skin, and the perineurium of peripheral nerves (3-7, 9, 10). The human LAMA4 cDNA encodes 1823 amino acids (aa), including a 24 aa signal sequence and a 1799 aa mature protein that contains three EGF-like repeats (aa 82-240) followed by five coiled-coil regions (aa 320-806) and five globular, laminin G-like (LG) domains (aa 833-1820). Potential isoforms of 1816 aa and 1791 aa are lacking sequences following the EGF-like repeats. A protease-sensitive region between LG3 and LG4 produces a processed ~180 kDa form that is detected in vivo (9, 10). Within the LG region, human and mouse LAMA4 share 91% aa sequence identity. LAMA4 LG domains serve as Ca⁺⁺-mediated receptors for integrins such as alpha v beta 3 and alpha 3 beta 1 (mainly at LG2), fibulin-1 and -2 (multiple LG), and heparan sulfate proteoglycans such as syndecans 2 and 4 (mainly at LG4) (8-12). Deletion of LAMA4 in mice results in misalignment of pre- and post-synaptic membranes at neuromuscular junctions, faulty nerve myelination, chronic kidney disease, deficient neutrophil extravasation, and microvessel instability, which causes cardiomyopathy and circulatory dysfunctions (5-8, 12-14). These results show the importance of LAMA4-containing laminins in normal synapse maturation and blood vessel maturation.

References

Tzu, J. and M.P. Marinkovich (2008) Int. J. Biochem. Cell Biol. 40:199.
Durbeej, M. (2010) Cell Tiss. Res. 339:259.
Liu, J. et al. (1996) Matrix Biol. 15:433.
Iivanainen, A. et al. (1997) J. Biol. Chem. 272:27862.
Patton, B.L. et al. (2001) Nat. Neurosci. 4:597.
Wallquist, W. et al. (2005) J. Neurosci. 25:3692.
Abrass, C.K. et al. (2010) Am. J. Pathol. 176:839.
Wondimu, Z. et al. (2004) Blood 104:1859.
Matsuura, H. et al. (2004) J. Invest. Dermatol. 122:614.
Talts, J.F. et al. (2000) J. Biol. Chem. 275:35192.
Gonzalez, A.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16075.
DeHahn, K.C. et al. (2004) Exp. Cell Res. 294:281.
Thyboll, J. et al. (2002) Mol. Cell Biol. 22:1194.
Wang, J. et al. (2006) J. Biol. Chem. 281:213.

Entrez Gene IDs

3910 (Human); 16775 (Mouse); 309816 (Rat)

Alternate Names

DKFZp686D23145; LAMA3LAMA4*-1; LAMA4; laminin alpha 4 chain; Laminin alpha 4; laminin subunit alpha-4; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha

Related Research Areas

Citations for Recombinant Human Laminin alpha 4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity
Authors: MA Gonzalez P, K Stojkova, MK Vaicik, A Pelowe, A Goddi, A Carmona, B Long, AA Qutub, A Gonzalez, RN Cohen, EM Brey
Scientific Reports, 2021-03-08;11(1):5442.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
LAMA4 upregulation is associated with high liver metastasis potential and poor survival outcome of Pancreatic Cancer
Authors: B Zheng, J Qu, K Ohuchida, H Feng, SJF Chong, Z Yan, Y Piao, P Liu, N Sheng, D Eguchi, T Ohtsuka, K Mizumoto, Z Liu, S Pervaiz, P Gong, M Nakamura
Theranostics, 2020-08-13;10(22):10274-10289.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Pathological Progression Induced by the Frontotemporal Dementia-Associated R406W Tau Mutation in Patient-Derived iPSCs
Authors: M Nakamura, S Shiozawa, D Tsuboi, M Amano, H Watanabe, S Maeda, T Kimura, S Yoshimatsu, F Kisa, CM Karch, T Miyasaka, A Takashima, N Sahara, SI Hisanaga, T Ikeuchi, K Kaibuchi, H Okano
Stem Cell Reports, 2019-09-19;13(4):684-699.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Direct contact with perivascular tumor cells enhances integrin ï¿½v?3 signaling and migration of endothelial cells
Authors: ME Burgett, JD Lathia, P Roth, AS Nowacki, DS Galileo, E Pugacheva, P Huang, A Vasanji, M Li, T Byzova, T Mikkelsen, S Bao, JN Rich, M Weller, CL Gladson
Oncotarget, 2016-07-12;7(28):43852-43867.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins.
Authors: Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
Matrix Biol, 2014-03-27;36(0):5-14.
Species: Human, Mouse
Sample Types: In Vivo, Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay, In Vivo