Recombinant Human KIR3DL1 His-tag Protein, CF

Catalog #: 11101-KR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11101-KR-050

Recombinant Human KIR3DL1 His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human KIR3DL1 His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED₅₀ for this effect is 0.0200-0.200 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human KIR3DL1 protein
His22-His340, with C-terminal 6-His tag

Accession #

P43629.1

N-terminal Sequence
Analysis

His22

Predicted Molecular Mass

36 kDa

SDS-PAGE

45-58 kDa, under reducing conditions.

Product Datasheets

11101-KR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11101-KR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED₅₀ for this effect is 0.0200-0.200 μg/mL.

N/A

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2 μg/lane of Recombinant Human KIR3DL1 His-tag Protein (Catalog # 11101-KR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-58 kDa.

Reconstitution Calculator

Background: KIR3DL1

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56^dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40 - 48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

References

Colonna, M. and J. Samaridis (1995) Science 268:405.
Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
Uhrberg, M. et al. (1997) Immunity 7:753.
O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
Thananchai, H. et al. (2007) J. Immunol. 178:33.
Martin, M.P. et al. (2007) Nat. Genet. 39:733.
Pando, M.J. et al. (2003) J. Immunol. 171:6640.
Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
Wilson, E.B. et al. (2007) Immunogenetics 59:641.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 1

Entrez Gene IDs

3811 (Human); 245616 (Mouse)

Alternate Names

AMB11; CD158 antigen-like family member E; CD158e antigen; CD158E; CD158e1; CD158e1/2; CD158e2; cl-11; cl-2; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3; killer Ig receptor; KIR antigen 3DL1; KIR3DL1; KIR3DL1/S1; KIR3DS1; MGC119726; MGC119728; MGC126589; MGC126591; MHC class I NK cell receptor; Natural killer-associated transcript 3; Nkat3; NKAT-3; NKAT3KIR; NKB1; NKB1B; NKB1CD158E1; NK-receptor; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11