Recombinant Human KGF/FGF-7 Protein, CF

Catalog #: 11340-KG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11340-KG-010
11340-KG-050
11340-KG-100
11340-KG-500
11340-KG-01M

Recombinant Human KGF/FGF‑7 Protein Bioactivity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human KGF/FGF-7 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 25.0-250 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human KGF/FGF-7 protein
Cys32-Thr194

Accession #

P21781.1

N-terminal Sequence
Analysis

Cys32

Predicted Molecular Mass

19 kDa

SDS-PAGE

22-28 kDa, under reducing conditions.

Product Datasheets

11340-KG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11340-KG

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
Reconstitution	Reconstitute 10 μg size at 100 μg/mL and all the other sizes at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human KGF/FGF-7 Protein (Catalog # 11340-KG) stimulates cell proliferation of MCF-7 human breast cancer cells, The ED₅₀ for this effect is 25.0-250 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # 11340-KG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-28 kDa.

Reconstitution Calculator

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4,13). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

References

Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
Mason, I.J. et al. (1994) Mech. Dev. 45:15.
Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
Qiao, J. et al. (1999) Development 126:547.
Guo, L. et al. (1996) Genes Dev. 10:165.
de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
Belleudi, F. et al. (2007) Traffic 8:1854.
Vadija R. et al. (2016) J Chem Biol. 9:69.

Long Name

Keratinocyte Growth Factor

Entrez Gene IDs

2252 (Human); 403915 (Canine)

Alternate Names

FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)