Recombinant Human Integrin alpha 4 beta 7/LPAM 1 Protein, CF

Catalog #: 11509-A3 Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
11509-A3-050

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All Integrin alpha 4 beta 7/LPAM-1 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Integrin alpha 4 beta 7/LPAM 1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 4 beta 7/LPAM-1 Fc Chimera (Catalog # 11509-A3) binds Recombinant Human MAdCAM-1 Fc Chimera (Catalog # 6056-MC) with an ED₅₀ of 30.0-450 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Integrin alpha 4 beta 7/LPAM-1 protein

Human ITGA4 (Tyr34-Gln970,R591L & R878Q) Accession # P13612.3	IEGR	Human IgG₁ (Glu99-Lys330) (with modifications)
Human ITGB7 (Glu20-His723) Accession # P26010.1	IEGR	Human IgG₁ (Glu99-Lys330) (with modifications)
N-terminus		C-terminus

Accession #

P13612.3 (Integrin alpha 4) & P26010.1 (Integrin b

N-terminal Sequence
Analysis

Tyr34 (Integrin alpha 4) & Glu20 (Integrin beta 7)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

130 kDa (Integrin alpha 4) & 104 kDa (Integrin beta 7)

SDS-PAGE

115-135 kDa (Integrin beta 7) & 140-160 kDa (Integrin alpha 4), under reducing conditions.

Product Datasheets

11509-A3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11509-A3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Human Integrin alpha 4 beta 7/LPAM-1 Fc Chimera Protein (Catalog # 11509-A3) binds Recombinant Human MAdCAM-1 Fc Chimera (6056-MC) with an ED₅₀ of 30.0‑450 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Integrin alpha 4 beta 7/LPAM‑1 Fc Chimera Protein (Catalog # 11509-A3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa (Integrin beta 7) & 140-160 kDa (Integrin alpha 4), under reducing conditions and 250-280 kDa under non-reducing conditions.

Reconstitution Calculator

Background: Integrin alpha 4 beta 7/LPAM-1

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 (1). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 150 kDa alpha 4 (CD49d) subunit and a 130 kDa beta 7 subunit (2, 3). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure followed by thigh, calf-1, and calf-2 domains (1). The beta 7 ECD contains a vWFA domain, which interacts with the
alpha 4 beta -propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The human alpha 4 ECD shares 85% amino acid sequence identity with the mouse, rat, and canine alpha 4 ECD. The human beta 7 ECD shares 88% and 87% amino acid sequence identity with the rat and mouse beta 7 ECD, respectively. Integrin alpha 4 beta 7 binds the mucosal addressin MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).

References

Luo, B.H. et al. (2007) Annu. Rev. Immunol. 25:619.
Takada, Y. et al. (1989) EMBO J. 8:1361.
Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
Chen, J. et al. (2004) J. Biol. Chem. 279:55556.
Park, E.J. et al. (2007) J. Clin. Invest. 117:2526.
Yu, Y. et al. (2012) J. Cell Biol. 196:131.
Yang, Y. et al. (1998) Eur. J. Immunol. 28:995.
Wagner, N. et al. (1996) Nature 382:366.
Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
Edele, F. et al. (2008) J. Immunol. 181:3745.
Arthos, J. et al. (2008) Nat. Immunol. 9:301.
Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
Monteiro, P. et al. (2011) J. Immunol. 186:4618.
Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha