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Recombinant Human Integrin a5b1 Protein, CF

Catalog #: 3230-A5B Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3230-A5B-050
Recombinant Human Integrin alpha 5 beta 1 Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Integrin a5b1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.20 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 (Catalog # 3230-A5B) binds Human Fibronectin (Catalog # 1918-FN) with an ED50 of 1.00-12.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
Human Integrin alpha 5
(Phe42-Tyr995)
Accession # P08648		Acidic Tail
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556		Basic Tail
N-terminus C-terminus
Accession #
P08648 & P05556
N-terminal Sequence
Analysis
Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)
Predicted Molecular Mass
108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit)
SDS-PAGE
110-140 kDa, under reducing conditions.

Product Datasheets

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3230-A5B

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3230-A5B

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Recombinant Human Integrin alpha 5 beta 1 Protein (Catalog # 3230-A5B) binds Human Fibronectin (1918-FN) with an ED50 of 1.00-12.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Integrin alpha 5 beta 1 Protein (Catalog # 3230-A5B) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-140 kDa, under reducing conditions..

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 5 beta 1

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

References
  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
Entrez Gene IDs
3678 (Human)
Alternate Names
CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 beta 1; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A

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