Recombinant Human IL-39 Fc Chimera Protein, CF

Catalog #: 9990-IL
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9990-IL-050

Recombinant Human IL-39 Fc Chimera Protein Bioactivity

2 Images

All IL-39 (IL-23p19/EBI3) Proteins and Enzymes

Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-39 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IFN-gamma production by mouse splenocytes. The ED₅₀ for this effect is 50-250 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-39 (IL-23p19/EBI3) protein

Human EBI3
(Arg21-Lys229, Gln95Cys)
Accession # Q14213

GGGSGGGSGGGS

Human IL-23p19
(Arg20-Pro189)
Accession # Q9NPF7

IEGRMD

Human IgG₁
(Pro100-Lys330)

Accession #

Q14213, Q9NPF7

N-terminal Sequence
Analysis

Arg21 (EBI3)

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

69 kDa

SDS-PAGE

70-77 kDa, reducing conditions

Product Datasheets

9990-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9990-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IL-39 Fc Chimera (Catalog # 9990-IL) induces IFN-gamma production in mouse splenocytes. The ED₅₀ for this effect is 50-250 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IL-39 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-77 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Background: IL-39 (IL-23p19/EBI3)

Interleukin 39 (IL-39) is a member of the IL-12 family of heterodimeric cytokines. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-39 are the IL-23 p19 subunit and the EBI3 subunit, respectively (1-3). The IL-23 p19 subunit of IL-39 is synthesized as a 189 amino acid (aa) precursor protein with a 19 aa signal sequence and a 170 aa mature region. The EBI3 subunit of IL-39 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-39 share 73% and 62% sequence homology in their IL-23 p19 and EBI3 subunits, respectively. Heterodimer (IL-39) is secreted by LPS-stimulated B cells and GL7(+) activated B cells of lupus-like mice (1, 4). IL-39 signals through IL-23 R/gp130 receptors and mediates inflammatory responses through activation of STAT1 and STAT3 in lupus-like mice (1, 2). IL‐39 induces and/or expands neutrophils in lupus‐prone mice, and IL‐39‐induced neutrophils increase the secretion of B cell activation factor (BAFF) (3). Thus, IL-39 is an important pro-inflammatory cytokine and play important roles in the pathophysiology of autoimmune diseases, including Systemic Lupus Erythematosus (SLE) (2, 4).

References

Wang, X. et al. (2016) Eur J Immunol 46:1343.
Hasegawa, H. et al. (2016) Front Immunol 7:479.
Wang, X. et al. (2016) Clin Exp Immunol 186:144.
Wang, X. et al. (2018) Mol Med Rep 17:1660.

Long Name

Interleukin 39 (IL-23p19/EBI3 Heterodimer)

Entrez Gene IDs

51561 (Human)

Alternate Names

IL-23 p19/EBI3; IL-23p19/EBI3; IL-39 (IL-23p19/EBI3)

Related Research Areas

Citations for Recombinant Human IL-39 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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IL-39 Reduces Proliferation and Promotes Apoptosis of Bladder Cancer by Altering the Activity of Cyclin E and Fas.
Authors: Xiao H, Alisic H, Reiman B, Deng Z, Zhu Z, Givens N, Bai Q, Tait A, Wakefield M, Fang Y
Anticancer Res, 2021-05-01;41(5):2239-2245.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Human neutrophils activated by TLR8 agonists, with or without IFNgamma, synthesize and release EBI3, but not IL-12, IL-27, IL-35, or IL-39
Authors: MA Cassatella, E Gardiman, F Arruda-Sil, F Bianchetto, S Gasperini, M Bugatti, W Vermi, F Larousseri, O Devergne, N Tamassia
J. Leukoc. Biol., 2020-06-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Reference Standard