Recombinant Human IL-2 Heat Stable Agonist Protein, CF New

Proliferation bioassay in NK92 cells after 59 days in media at 37 °C. Higher stability allows for less dosing throughout extended T cell cultures as well as early media prep to minimize culture inconsistencies from degrading cytokines.

After 10 min at 95°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >14-fold activity.

After 10 days at 10 ng/mL in the cell culture medium at 37°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >3-fold activity.

Recombinant Human IL 2 Heat Stable Agonist (Catalog # BT-002HS) stimulates proliferation of NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.0150‑0.150 ng/mL.

2 μg/lane of Recombinant Human IL‑2 Heat Stable Agonist Protein (Catalog # BT-002HS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 7 kDa.

Heat Stable IL-2 (BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3-, anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein.

CD4+ and CD8+ T cells were activated with anti-CD3-, anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein.