Recombinant Human IL-15R alpha Fc Chimera Protein, CF Summary
Product Specifications
| Human IL-15 R alpha (Ile31-Thr172) Accession # EAW86418 |
IEGRDMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
147-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-15R alpha
Interleukin 15 Receptor alpha (IL‑15 R), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature human IL‑15 R alpha consists of a 175 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 23 aa transmembrane segment, and a 39 aa cytoplasmic tail (4). Within the ECD, human IL‑15 R alpha shares approximately 60% aa sequence identity with mouse and rat IL‑15 R alpha. Alternate splicing of human IL‑15 R alpha generates additional isoforms with variable length deletions in the ECD and/or substitutions in the cytoplasmic domain (4, 5). IL‑15 R alpha binds to Interleukin‑15 with high affinity (6). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (7, 8). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (10 ‑ 12). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (13, 14). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (6, 15, 16).
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
- Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
- Giri, J.G. et al. (1995) EMBO 14:3654.
- Grabstein, K. et al. (1994) Science 264:965.
- Giri, J. et al. (1994) EMBO J. 13:2822.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
- Dubois, S. et al. (2002) Immunity 17:537.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
- Neely, G.G. et al. (2004) J. Immunol. 172:4225.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
- Mortier, E. et al. (2004) J. Immunol. 173:1681.
Citations for Recombinant Human IL-15R alpha Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
The IL-15 / sIL-15R&alpha complex modulates immunity without effect on asthma features in mouse
Authors: A Moui, M Klein, D Hassoun, E Dijoux, MA Cheminant, A Magnan, G Bouchaud
Respir. Res., 2020-01-29;21(1):33.
Species: Mouse
Sample Types: In Vivo
-
Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence
Authors: L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini
Cell Rep, 2020-01-21;30(3):687-698.e6.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes
Authors: RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi
PLoS Pathog, 2016-04-15;12(4):e1005545.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation.
Authors: Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont
Scientific Reports, 2016-02-26;0(0):2045-2322.
Species: Xenograft
Sample Types: In Vivo
Applications: In Vivo -
IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
PLoS ONE, 2012-07-09;7(7):e40620.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops.
Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E
J. Immunol., 2005-09-15;175(6):3656-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
J. Immunol., 2004-07-15;173(2):1463-76.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human IL-15R alpha Fc Chimera Protein, CF
Average Rating: 4.7 (Based on 3 Reviews)
Have you used Recombinant Human IL-15R alpha Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
We used this protein in an in-house ELISA along with mAb and pAb (AF247) to quantify IL-15R alpha in human serum and plasma. This combination could not detect IL-15R alpha in our samples but generated a good standard curve.
