Recombinant Human IL-12/IL-23 p40 Protein, CF
Recombinant Human IL-12/IL-23 p40 Protein, CF Summary
Product Specifications
Ile23-Ser328
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11407-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute 10 μg size at 100 μg/mL and other sizes at 500 μg/mLin PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human IL-12/IL-23 p40 Protein (Catalog # 11407-IL) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 2.00-20.0 ng/mL.
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2 μg/lane of Recombinant Human IL‑12/IL‑23 p40 Protein (Catalog # 11407-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-45 kDa, under reducing conditions.
Reconstitution Calculator
Background: IL-12/IL-23 p40
Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions (1). It is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human IL-12p40 is a 40 kDa glycoprotein that shows considerable structural similarity to the extracellular domain of hematopoietin receptors (2). It is synthesized as a 328 amino acid (aa) precursor with a 22 aa signal sequence and a 306 aa mature region that contains a 92 aa fibronectin type III domain and an 84 aa Ig C2-like region and has a high degree of structural homology to type I cytokine receptors. There are two intrachain disulfide bonds and four potential N-linked glycosylation sites (3). Once made, it can exist in multiple forms including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12) (1, 4, 5). Mature human IL-12p40 shows 66% aa sequence identity to mouse and rat IL-12p40 respectively. The secreted form of the p40 subunit inhibits IL-23 functions and abrogates IL-23-mediated anti-tumor effects (6). Characterization of the IL-12p40 proteins for binding and bioactivity showed that both the p40 monomer and dimer inhibited IL-12 binding to IL-12R (7).
- Trinchieri, G. et al. (2003) Immunity 19:641.
- Egwuagu, C. E. et al. (2015) Cytokine Growth Factor Rev. 26:587.
- Tone, Y. et al. (1996) Eur. J. Immunol. 26:1222.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Oppmann,B. et al. (2000) Immunity 13:715.
- Shimozato, O et al. (2005) Immunology 117:22.
- Ling, P et al. (1995) J. Immunol. 154:116.
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