Recombinant Human IL-12/IL-23 p40 Monomer Protein

Catalog #: 309-IL
6 Citations Datasheet / COA / SDS

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Recombinant Human IL-12/IL-23 p40 Monomer Protein Binding Activity

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Citations (6)

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Recombinant Human IL-12/IL-23 p40 Monomer Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human IL‑12/IL‑23 p40 Monomer that produces 50% of the optimal binding response is 1-6 ng/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-12/IL-23 p40 protein
Ile23-Ser328

Accession #

P29460

N-terminal Sequence
Analysis

Ile23

Predicted Molecular Mass

34.7 kDa

SDS-PAGE

40 kDa, reducing conditions

Product Datasheets

309-IL

Product Datasheet

COA

SDS

309-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Monomer (Catalog # 309-IL) binds with an ED₅₀ of 1-6 ng/mL.

Reconstitution Calculator

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

Related Research Areas

Citations for Recombinant Human IL-12/IL-23 p40 Monomer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12R&beta1 internalization and suppress EAE
Authors: S Mondal, M Kundu, M Jana, A Roy, SB Rangasamy, KK Modi, J Wallace, YA Albalawi, R Balabanov, K Pahan
Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;117(35):21557-21567.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
The localisation of the heparin binding sites of human and murine interleukin-12 within the carboxyterminal domain of the P40 subunit
Authors: P Garnier, R Mummery, MJ Forster, B Mulloy, RV Gibbs, CC Rider
Cytokine, 2018-05-10;110(0):159-168.
Species: Human
Sample Types: Complex Sample Type
Applications: Bioassay
Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
Authors: Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H
J Immunol, 2014-05-12;192(12):6028-36.
Species: Mouse
Sample Types:
Applications: Bioassay
Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
J. Allergy Clin. Immunol., 2006-06-21;118(2):354-60.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
J. Immunol., 2006-06-15;176(12):7768-74.
Applications: ELISA (Specificity Testing)
Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
J. Neuroimmunol., 2006-03-29;175(1):169-75.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)