Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

Catalog #: 1241-S8 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1241-S8-050

Recombinant Human IGSF8/CD316 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IGSF8/CD316 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant CD81 Fc Chimera binds with an ED₅₀ of 1.2-7.2 μg/mL

Source

Human embryonic kidney cell, HEK293-derived human IGSF8/CD316 protein

Human IGSF8 (Arg28-Thr579) Accession # Q969P0-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q969P0-1

N-terminal Sequence
Analysis

Arg28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

85 kDa

SDS-PAGE

86-113 kDa, reducing conditions

Product Datasheets

1241-S8

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1241-S8

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IGSF8/CD316 Fc Chimera (Catalog # 1241-S8) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant CD81 Fc Chimera binds with an ED₅₀ of 1.2-7.2 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IGSF8/CD316 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-113 kDa and 170-230 kDa, respectively.

Reconstitution Calculator

Background: IGSF8/CD316

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha 3 beta 1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha -Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

References

Clark, K.L. et al. (2001) J. Immunol. 167:5115.
Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
Charrin, S. et al. (2003) Biochem. J. 373:409.
Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
Kolesnikova, T. et al. (2009) Neoplasia.11:77.

Long Name

Immunoglobulin Superfamily, Member 8

Entrez Gene IDs

93185 (Human); 140559 (Mouse)

Alternate Names

CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8