Recombinant Human IFN-alpha 21/IFNA21 Protein, CF

Catalog #: 11159-IF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11159-IF-010
11159-IF-050

Recombinant Human IFN-alpha 21/IFNA21 Protein Bioactivity.

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All IFN-alpha 21/IFNA21 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-alpha 21/IFNA21 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 1.00-30.0 pg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-alpha 21/IFNA21 protein
Cys24-Glu189

Accession #

P01568.2

N-terminal Sequence
Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Product Datasheets

11159-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11159-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IFN-alpha 21/IFNA21 Protein (Catalog # 11159-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 1.00-30.0 pg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IFN-alpha 21/IFNA21 Protein (Catalog # 11159-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa, under reducing conditions.

Reconstitution Calculator

Background: IFN-alpha 21/IFNA21

Interferons (IFN) are a family of cytokines with potent antiviral, anti-proliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). There is not a mouse homolog for IFNA21, but mature human IFNA21 is identical to chimpanzee IFNA21. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, with IFNA21 displaying intermediate activity inducing interferon stimulating genes (8). Further, human IFNA21 has shown weak anti-viral activity against viruses such as metapneumovirus (9).

References

Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
Goeddel, D.V. et al. (1980) Nature 287:411.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
van Pesch, V. et al. (2004) J. Virol. 78:8219.
James, C.M. et al. (2007) Vaccine. 25(10):1856.
Moll, H.P. et al. (2011) Cytokine. 53:52.
George, J. and Mattapallil, J.J. (2018) Frontiers in immunology 9:299.

Long Name

Interferon alpha F

Entrez Gene IDs

3452 (Human)

Alternate Names

IFNA21; IFN-alpha 21; IFNalpha F; IFN-alpha F; IFN-alpha-21; IFN-alphaI; interferon alpha-21; Interferon alpha-F; interferon, alpha 21; LeIF F; leukocyte interferon protein; MGC126687; MGC126689