Home / ICAM-3/CD50 / Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

Catalog #: 715-IC
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
715-IC-050
715-IC-200
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 40-70% will adhere after PMA stimulation for 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-3/CD50 protein
Human ICAM-3
(Gln30 - His485)
Accession # CAA49473		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
CAA49473
N-terminal Sequence
Analysis
No results obtained: Gln30 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
76.7 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions

Product Datasheets

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715-IC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

715-IC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References
  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Entrez Gene IDs
3385 (Human)
Alternate Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

Citations for Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Integrin alphaDbeta2 (CD11d/CD18) is expressed by human circulating and tissue myeloid leukocytes and mediates inflammatory signaling.
    Authors: Miyazaki Y, Vieira-de-Abreu A, Harris E, Shah A, Weyrich A, Castro-Faria-Neto H, Zimmerman G
    PLoS ONE, 2014-11-21;9(11):e112770.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
    Authors: Wu L, 107253, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN
    IL-6 signalling biomarkers in hospitalised patients with moderate to severe SARS-CoV-2 infection in a single centre study in Sweden, 2002-01-29;99(3):1568-73.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Soluble adhesion molecules in juvenile rheumatoid arthritis.
    Authors: Bloom BJ, Miller LC, Tucker LB, Schaller JG, Blier PR
    J. Rheumatol., 1999-09-01;26(9):2044-8.
    Applications: ELISA (Standard)

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