Recombinant Human HMGN1 Protein, CF Summary
Product Specifications
Pro2-Asp100
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8187-HM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: HMGN1
High-mobility group nucleosome-binding protein 1 (HMGN1), also known as HMG-14, is a member of the family of non-histone chromosomal proteins that play important roles in chromatin remodeling (1, 2). HMGN1 contains an N-terminal domain that interacts with nucleosomes and a C-terminal domain that assists chromatin unfolding (2, 3). Human HMGN1 shares 80% amino acid sequence identity with mouse and rat HMGN1. Alternative RNA processing generates a long isoform with a 45 amino acid insertion following Met1 (4). The trafficking of HMGN1 between the nucleus and cytoplasm is regulated by its serine phosphorylation at multiple sites (5, 6). In the nucleus, HMGN1 preferentially associates with sites of chromatin remodeling and gene transcription, allowing it to modulate the expression of a wide variety of genes (7, 8). It contributes to DNA damage repair by directly associating with and activating the double strand break sensor PARP-1 (9). HMGN1 is additionally one of several alarmins, proteins that are normally intracellular but are released following tissue damage or necrosis (1). In this setting, HMGN1 promotes dendritic cell maturation, recruitment to sites of inflammation, and production of Th1 inflammatory cytokines (10). These pro-inflammatory activities are dependent on the direct association of HMGN1 with the TLR4-MD2 complex on dendritic cells (10, 11).
- Yanai, H. et al. (2012) Trends Immunol. 33:633.
- Gerlitz, G. (2010) Biochim. Biophys. Acta 1799:80.
- Landsman, D. et al. (1986) J. Biol. Chem. 261:16082.
- Zougman, A. et al. (2008) Curr. Biol. 18:1760.
- Louie, D.F. et al. (2000) Protein Sci. 9:170.
- Pogna, E.A. et al. (2010) Biochim. Biophys. Acta 1799:93.
- Cuddapah, S. et al. (2011) Mol. Cell. Biol. 31:700.
- Kugler, J.E. et al. (2013) J. Biol. Chem. 288:16690.
- Masaoka, A. et al. (2012) J. Biol. Chem. 287:27648.
- Yang, D. et al. (2010) Biochim. Biophys. Acta 1799:157.
- Yang, D. et al. (2012) J. Exp. Med. 209:157.
Citation for Recombinant Human HMGN1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Alpha synuclein, the culprit in Parkinson disease, is required for normal immune function
Authors: MM Alam, Yang, XQ Li, J Liu, TC Back, A Trivett, B Karim, D Barbut, M Zasloff, JJ Oppenheim
Cell Reports, 2022-01-11;38(2):110090.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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