Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-1, CF

Catalog #: 5057-ST
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5057-ST-020

R&D Systems Recombinant Proteins and Enzymes

1 Image

All Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Proteins and Enzymes

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-1, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is >45 pmol/min/μg, as measured under the described conditions.

Source

Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 protein
Pro28-Trp401, with an N-terminal 6-His tag

Accession #

AAY14736

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

45 kDa

SDS-PAGE

56-60 kDa, reducing conditions

Product Datasheets

5057-ST

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5057-ST

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials

Assay Buffer: 50 mM Tris, 15 mM MgCl₂, pH 7.5
Recombinant Human Heparan Sulfate 6‑O‑Sulfotransferase 1/HS6ST1 (rhHS6ST1) (Catalog # 5057-ST)
Donor Substrate: 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) (Catalog # ES019)
Acceptor Substrate: Heparan Sulfate (Celsus Labs, Catalog # HO3105), 50 mg/mL stock in deionized water
Sulfotransferase Activity Kit (Catalog # EA003)
96-well Clear Plate (Costar, Catalog # 92592)
Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent

Create Assay Buffer by Diluting 10X Phosphatase Buffer 3 (supplied in kit) to 1X in deionized water.
Dilute 1 mM Phosphate Standard by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 µM stock.
Continue standard curve by performing six one-half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5 nmol per well.
Prepare a reaction mixture containing 0.4 mM PAPS, 4 mg/mL Heparan Sulfate, and 20 ug/mL Coupling Phosphatase 3 in Assay Buffer. *Note: Incorporate Coupling Phoshphatase 3 into reaction mixture right before the mixture is added to the plate in order to avoid unnecessary hydrolysis of PAPS.
Dilute rhHS6ST1 to 20 µg/mL in Assay Buffer.
Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
Load 25 µL of the 40 µg/mL rhHS6ST1 into the plate. Include a control containing 25 µL of Assay Buffer.
Add 25 µL of reaction mixture to the wells, excluding the standard curve.
Seal plate and incubate at 37 °C for 20 minutes.
Add 30 µL of the Malachite Green Reagent A to all wells.
Add 100 µL of deionized water to all wells. Mix briefly.
Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
Read plate at 620 nm (absorbance) in endpoint mode.
Calculate specific activity:

Specific Activity (pmol/min/µg) =	Phosphate released* (nmol) x (1000 pmol/nmol)
	Incubation time (min) x amount of enzyme (µg)

*Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

Per Reaction:

rhHS6ST1: 0.5 µg
Coupling Phosphatase 3: 0.5 µg
Heparan Sulfate: 100 ug (2 mg/mL)
PAPS: 0.2 mM

Reconstitution Calculator

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1, 2). Heparan Sulfate consists of repeating residues of uronic acid and N‑acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2‑O‑Sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned (3). Mice deficient of the HS6ST1 homologue gene showed embryonic lethality (4).The enzymatic activity of the recombinant human HS6ST1 is measured using a phosphatase-coupled assay (5).

References

Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.
Habuchi, H et al. (1998) J. Biol. Chem. 273:9208.
Habuchi, H et al. (2007) J. Biol. Chem. 282:15578.
Prather, B. et al. (2012) Anal. Biochem. 423:86.

Entrez Gene IDs

9394 (Human); 50785 (Mouse); 316325 (Rat)

Alternate Names

DKFZp547H098; EC 2.8.2; EC 2.8.2.-; FLJ25392; heparan sulfate 6-O-sulfotransferase 1; heparan-sulfate 6-sulfotransferase; HS6ST1; HS6ST-1; HS6STheparan-sulfate 6-O-sulfotransferase 1; MGC116899; MGC116901

Related Research Areas

Citations for Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-1, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Structural and functional study of D-glucuronyl C5-epimerase.
Authors: Qin Y, Ke J, Gu X, Fang J, Wang W, Cong Q, Li J, Tan J, Brunzelle J, Zhang C, Jiang Y, Melcher K, Li J, Xu H, Ding K
J Biol Chem, 2015-01-07;290(8):4620-30.
Species: Zebrafish
Sample Types: Protein
Applications: Bioassay
Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
Authors: Wu ZL, Prather B, Ethen CM
Glycobiology, 2010-12-17;21(5):625-33.
Species: Bovine, Porcine
Sample Types: Heparin, Protein
Applications: Enzyme Assay