Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

Catalog #: 2157-GC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2157-GC-050

Recombinant Human Guanylyl Cyclase C His-tag Protein SDS-PAGE

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All Guanylyl Cyclase C/GUCY2C Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Guanylyl Cyclase C His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Source

Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Ser21-Gln430, with a C-terminal 6-His tag

Accession #

P25092.2

N-terminal Sequence
Analysis

Ser21

Predicted Molecular Mass

47 kDa

SDS-PAGE

73-81 kDa, reducing conditions

Product Datasheets

2157-GC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2157-GC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human Guanylyl Cyclase C/GUCY2C was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-81 kDa.

Reconstitution Calculator

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
Lucas K. et al. (2000) Pharmacol Rev. 52:375.
Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
Gong, R. et al. (2011) Science. 333:1642.
Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Alternate Names

DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor