Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

Catalog #: AVI2157 Datasheet / COA / SDS

Biotinylated /GUCY2C His-tag

Catalog #	Availability	Size / Price	Qty
AVI2157-050

Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein Binding Activity.

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All Guanylyl Cyclase C/GUCY2C Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody (Catalog # MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED₅₀ of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Source

Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein

Human Guanylyl Cyclase C Protein (Ser24-Gln430) Accession # P25092.2	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

P25092.2

N-terminal Sequence
Analysis

Ser24

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

49 kDa

SDS-PAGE

75-85 kDa, under reducing conditions.

Product Datasheets

AVI2157

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI2157

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human Guanylyl Cyclase C Antibody (MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag (Catalog # AVI2157) binds with an ED₅₀ of 0.250-1.50 ng/mL

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein (Catalog # AVI2157) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa.

Reconstitution Calculator

Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1 - 4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Arshad, N. and Visweswariah S.S. (2012) FEBS Lett. 586:2835.
Potter, L.R. (2011) Cell Signal (2011) 23:1921.
Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
Lucas, K. et al. (2000) Pharmacol Rev. 52:375.
Gong, R. et al. (2011) Science 333:1642.
Waldman, S.A and Camilleri, M. (2018) Gut 67:1543.
Kim, G.W. et al. (2013) Trends Endocrinol Metab 24(4):165.

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Alternate Names

DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor