Recombinant Human Flt-3/Flk-2 His-tag Protein, CF

Catalog #: 10284-F3 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10284-F3-050

Recombinant Human Flt-3/Flk-2 His-tag Protein Binding Activity

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Flt-3/Flk-2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Flt‑3/Flk‑2 His-tag is captured onto a His Tag Antibody (Catalog # MAB050R) coated plate, Recombinant Human Flt-3 Ligand (Catalog # 308-FK) binds with an ED₅₀ of 2-12 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Flt-3/Flk-2 protein
Asn27-Asn541, with a C-terminal 6-His tag

Accession #

P36888

N-terminal Sequence
Analysis

Asn27

Predicted Molecular Mass

59 kDa

SDS-PAGE

82-93 kDa, reducing conditions

Product Datasheets

10284-F3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10284-F3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Recombinant Human Flt-3/Flk-2 His-tag (Catalog # 10284-F3) is captured onto a His Tag Antibody (Catalog # MAB050R) coated plate, Recombinant Human Flt-3 Ligand (Catalog # 308-FK) binds with an ED₅₀ of 2-12 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human Flt-3/Flk-2 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 80-95 at kDa.

Reconstitution Calculator

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF (1). The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) type I membrane protein with a 26 aa signal peptide, a 515 aa extracellular domain (ECD) with 10 potential N-linked glycosylation sites, a 21 aa transmembrane domain and a 431 aa cytoplasmic domain. Within ECD human Flt-3 shares 85% and 82% aa sequence identity with mouse and rat Flt-3, respectively. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist (2).

References

Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Alternate Names

CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3