Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Catalog #: 368-ST
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
368-ST-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
368-ST-050

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (5)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Flt-3/Flk-2 Fc Chimera Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. The ED₅₀ for this effect is 0.01-0.03 µg/mL in the presence of rhFlt-3 Ligand at 2 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human Flt-3/Flk-2 protein

Human Flt-3 (Asn27-Asn541) Accession # AAA18947	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

AAA18947

N-terminal Sequence
Analysis

Asn27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

85.5 kDa (monomer)

SDS-PAGE

120 kDa, reducing conditions

Product Datasheets

368-ST (with carrier)

Product Datasheet

COA

SDS

368-ST/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

368-ST

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

368-ST/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References

Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Alternate Names

CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3

Related Research Areas

Citations for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain
Authors: A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat
Progress in neurobiology, 2023-01-13;0(0):102405.
Species: Human
Sample Types: Protein
Applications: Bioassay, SPR
Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution
Authors: Q Jiang, J Isquith, L Ladel, A Mark, F Holm, C Mason, Y He, P Mondala, I Oliver, J Pham, W Ma, E Reynoso, S Ali, IJ Morris, R Diep, C Nasamran, G Xu, R Sasik, SB Rosenthal, A Birmingham, S Coso, G Pineda, L Crews, ME Donohoe, JC Venter, T Whisenant, RA Mesa, LB Alexandrov, KM Fisch, C Jamieson
Cell Reports, 2021-01-26;34(4):108670.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
A systems biology pipeline identifies regulatory networks for stem cell engineering
Authors: MA Kinney, LT Vo, JM Frame, J Barragan, AJ Conway, S Li, KK Wong, JJ Collins, P Cahan, TE North, DA Lauffenbur, GQ Daley
Nat. Biotechnol., 2019-07-01;37(7):810-818.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation
Authors: Y Heshmati, S Kharazi, G Türköz, D Chang, E Kamali Dol, J Boström, A Krstic, T Boukoura, E Wagner, N Kadri, R Månsson, M Altun, H Qian, J Walfridsso
Sci Rep, 2018-07-25;8(1):11202.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34(+) cells.
Authors: Donahue RE, Wersto RP, Allay JA, Agricola BA, Metzger ME, Nienhuis AW, Persons DA, Sorrentino BP
Blood, 2000-01-15;95(2):445-52.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay