Recombinant Human Fibromodulin/FMOD Protein, CF

Catalog #: 9840-FM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9840-FM-050

Recombinant Human Fibromodulin/FMOD Protein Binding Activity

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All Fibromodulin/FMOD Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fibromodulin/FMOD Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Recombinant Human Fibromodulin/FMOD (Catalog # 9840-FM) binds with an ED₅₀ of 0.250-2.25 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human Fibromodulin/FMOD protein
Asp75-Ile376, with an N-terminal 10-His tag

Accession #

Q06828

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

36 kDa

SDS-PAGE

41-55 kDa, reducing conditions

Product Datasheets

9840-FM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9840-FM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Recombinant Human Fibromodulin/FMOD Protein (Catalog # 9840-FM) binds with an ED₅₀ of 0.250-2.25 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Fibromodulin/FMOD Protein (Catalog # 9840-FM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-55 kDa.

Reconstitution Calculator

Background: Fibromodulin/FMOD

Fibromodulin (FMOD) is a secreted, 60-70 kDa class II member of the small leucine-rich proteoglycan (SLRP) family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta (1, 2). FMOD is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers (3). Mature human FMOD is 358 amino acids (aa) in length (aa 19-376). It contains a sulfated N-terminal complement C1q binding domain (aa 19-98) and 12 Leu-rich repeats (LRRs) (aa 98-376), with the LRR5-7 and LRR11 responsible for collagen type I binding (2 - 4). Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation (5). Over aa 76-376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin. Recent study has shown that FMOD can induce glioma cell migration and invasion by promoting actin cytoskeleton remodeling (6).

References

Velez-Delvalle, C. et al. (2008) Biochem. Biophys. Res. Commun. 371:420.
Kalamajski, S. et al. (2007) J. Biol. Chem. 282:26740.
Kalamajski, S. et al. (2009) J. Biol. Chem. 284:534.
Sjoberg, A. et al. (2005) J. Biol. Chem. 280:32301.
Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26.
Mondal, B. et al. (2017) Oncogene 36:71.

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Alternate Names

Collagen-binding 59 kDa protein; fibromodulin proteoglycan; Fibromodulin; FM; FMOD; Keratan sulfate proteoglycan fibromodulin; SLRR2E; SLRR2EKSPG fibromodulin