Recombinant Human FGFR2 beta (IIIc) Fc Chimera Protein, CF

Catalog #: 684-FR
4 Citations Datasheet / COA / SDS

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684-FR-050

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR2 beta (IIIc) Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1‑3 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human FGF R2 beta protein

Human FGF R2 beta (IIIc) (Arg22 - Glu288)& (Leu27 - Glu288) Accession # NP_001138387	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_001138387

N-terminal Sequence
Analysis

Arg22 & Leu27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

100 kDa, reducing conditions

Product Datasheets

684-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

684-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FGFR2 beta

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References

Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 2 beta

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Alternate Names

FGF R2b; FGFR2 beta

Related Research Areas

Citations for Recombinant Human FGFR2 beta (IIIc) Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Clinical difference between fibroblast growth factor receptor 2 subclass, type IIIb and type IIIc, in gastric cancer
Authors: M Yashiro, K Kuroda, G Masuda, T Okuno, Y Miki, Y Yamamoto, T Sera, A Sugimoto, S Kushiyama, S Nishimura, S Togano, M Ohira
Scientific Reports, 2021-02-25;11(1):4698.
Species: Rat
Sample Types: In Vivo
Applications: Immunization
Pingyangmycin inhibits glycosaminoglycan sulphation in both cancer cells and tumour tissues
Authors: Y Lan, X Li, Y Liu, Y He, C Hao, H Wang, L Jin, G Zhang, S Zhang, A Zhou, L Zhang
J. Cell. Mol. Med., 2020-02-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline.
Authors: Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T
PLoS ONE, 2015-06-15;10(6):e0130147.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
FGF8 dose-dependent regulation of embryonic submandibular salivary gland morphogenesis.
Authors: Jaskoll T, Witcher D, Toreno L, Bringas P, Moon AM, Melnick M
Dev. Biol., 2004-04-15;268(2):457-69.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay