Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF

Catalog #: 661-FR
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
661-FR-050

Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein Bioactivity

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Product Details

Citations (9)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1‑3 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human FGF R1 beta protein

Human FGF R1 beta (IIIc) (Arg22-Glu285) Accession # NP_075594	IEGRDMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_075594

N-terminal Sequence
Analysis

Arg22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

90-95 kDa, reducing conditions

Product Datasheets

661-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

661-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human FGF R1 beta (IIIc) (Catalog # 661-FR) inhibits FGF acidic-dependent cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED₅₀ for this effect is 1-3 ng/mL.

SDS-PAGE

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1 μg/lane of Recombinant Human FGF R1 beta (IIIc) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90 kDa and 180 kDa, respectively.

Reconstitution Calculator

Background: FGFR1 beta

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1-3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References

Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 1 beta

Entrez Gene IDs

2260 (Human)

Alternate Names

FGF R1b; FGFR1 beta

Related Research Areas

Citations for Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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NCAM2 Fibronectin type-III domains form a rigid structure that binds and activates the Fibroblast Growth Factor Receptor
Authors: KK Rasmussen, MH Falkesgaar, M Winther, NK Roed, CL Quistgaard, MN Teisen, SM Edslev, DL Petersen, A Aljubouri, C Christense, PW Thulstrup, L Lo Leggio, K Teilum, PS Walmod
Sci Rep, 2018-06-12;8(1):8957.
Applications: Bioassay
A biparatopic agonistic antibody that mimics fibroblast growth factor 21 ligand activity
Authors: SY Shi, YW Lu, Z Liu, J Stevens, CM Murawsky, V Wilson, Z Hu, WG Richards, ML Michaels, J Zhang, W Yan, Y Li
J. Biol. Chem., 2018-02-26;0(0):.
Applications: Bioassay
Expression, refolding and spectroscopic characterization of fibronectin type III (FnIII)-homology domains derived from human fibronectin leucine rich transmembrane protein (FLRT)-1, -2, and -3
Authors: L Yang, M Hansen Fal, PW Thulstrup, PS Walmod, L Lo Leggio, K Krighaar R
PeerJ, 2017-07-07;5(0):e3550.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms.
Authors: Lelliott C, Ahnmark A, Admyre T, Ahlstedt I, Irving L, Keyes F, Patterson L, Mumphrey M, Bjursell M, Gorman T, Bohlooly-Y M, Buchanan A, Harrison P, Vaughan T, Berthoud H, Linden D
PLoS ONE, 2014-11-26;9(11):e112109.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay
Selection and characterization of a human neutralizing antibody to human fibroblast growth factor-2.
Authors: Tao J, Xiang JJ, Li D, Deng N, Wang H, Gong YP
Biochem. Biophys. Res. Commun., 2010-03-17;394(3):767-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.
Authors: Nakayama T, Mutsuga N, Tosato G
Blood, 2006-10-31;109(4):1363-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
A specific survival response in dopamine neurons at most risk in Parkinson's disease.
Authors: Murase S, McKay RD
J. Neurosci., 2006-09-20;26(38):9750-60.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
Development, 2005-02-16;132(6):1223-34.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signalling pathways in neocortical precursors.
Authors: Kessaris N, Jamen F, Rubin LL, Richardson WD
Development, 2004-02-11;131(6):1289-98.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay