Recombinant Human FGF-18 Protein, CF

Catalog #: 8988-F18
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8988-F18-050

Recombinant Human FGF-18 Protein Bioactivity

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Product Details

Citations (3)

Supplemental Products

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-18 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED₅₀ for this effect is 4-24 ng/mL

Source

E. coli-derived human FGF-18 protein
Glu28-Ala207, with a N-terminal Met

Accession #

O76093

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

21 kDa

SDS-PAGE

19-24 kDa, reducing conditions

Product Datasheets

8988-F18

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8988-F18

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3 mouse pro-B cells transfected with human FGF RIIIc. The ED₅₀ for this effect is 4-24 ng/mL.

Reconstitution Calculator

Background: FGF-18

Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).

References

Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
Miyaoka, Y. et al. (2010) Development 137:159.
Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
Liu, Z. et al. (2007) Dev. Biol. 302:80.
Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.

Long Name

Fibroblast Growth Factor 18

Entrez Gene IDs

8817 (Human)

Alternate Names

FGF18; FGF-18; FGF-18ZFGF5; fibroblast growth factor 18; zFGF5

Related Research Areas

Citations for Recombinant Human FGF-18 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats
Authors: I Carmon, R Smoum, E Farhat, E Reich, L Kandel, Z Yekhtin, R Gallily, R Mechoulam, M Dvir-Ginzb
Cells, 2022-12-16;11(24):.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
Amniotic suspension allograft improves pain and function in a rat meniscal tear-induced osteoarthritis model
Authors: KA Kimmerling, AH Gomoll, J Farr, KC Mowry
Arthritis Research & Therapy, 2022-03-04;24(1):63.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
Cell Stem Cell, 2020-02-20;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture