Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93)

Catalog #: 351-FS
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
351-FS-010/CF
351-FS-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
351-FS-010
351-FS-050

R&D Systems Recombinant Proteins and Enzymes

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All CXCL12/SDF-1 beta Proteins and Enzymes

Product Details

Citations (9)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93) Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is 10-30 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED₅₀ for this effect is 0.3-1.5 ng/mL.

Source

E. coli-derived CXCL12/SDF-1 beta protein
Lys22-Met93

Accession #

P48061

N-terminal Sequence
Analysis

Lys22

Predicted Molecular Mass

8 kDa

Product Datasheets

351-FS (with carrier)

Product Datasheet

COA

SDS

351-FS/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

351-FS

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

351-FS/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CXCL12/SDF-1 beta

CXCL12, also known as SCYB12, PBSF and SDF-1 beta, is an 8.3 kDa, heparin-binding member of the CXC (or alpha-) family of chemokines (1, 2). Feline CXCL12( beta ) is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region (3). The mature molecule exhibits a typical three antiparallel beta -strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa’s 1-8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation (4). The C-terminus is likely associated with heparin binding (5). SDF-1 beta circulates and undergoes proteolytic processing. CD26 will remove the first two N-terminal amino acids, possibly creating a reduced-activity chemokine (5, 6). In addition to the beta -isoform, alternate splicing of the feline SDF-1 gene generates an alpha -isoform. The alpha isoform is identical to SDF-1 beta, but shorter by four aa’s at the C-terminus (3). Although alpha - and beta -isoforms show similar activity, SDF-1 alpha is differentially processed, and different cells secrete the two isoforms (5, 7). Mature feline SDF-1 beta is 96%, 97% and 100% aa identical to rat, mouse and human SDF-1 beta, respectively. Human (and by inference, feline) SDF-1 is active on mouse cells. SDF-1 alpha and beta are reported to be monomers at neutral pH and physiologic ionic strength (4). SDF-1 alpha is also reported to form dimers in the presence of heparansulfate (8). On the cell surface, this may well facilitate SDF-1 interaction with its two receptors, CXCR4 and syndecan-4 (9). Heparin sulfate is known to protect SDF-1 from proteolysis, and CXCR4 exists constitutively as a dimer (9-11). Among its many functions, CXCL12 is known to influence lymphopoiesis, regulate patterning and cell number of neural progenitors, and promote angiogenesis (12, 13). It also enhances the survival of myeloid progenitor cells.

References

Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
Rollins, B.J. (1997) Blood 90:909.
Nishimura, Y. et al. (1998) Eur. J. Immunogenet. 25:303.
Crump, M.P. et al. (1997) EMBO J. 16:6996.
Sierra, M.D. L. et al. (2004) Blood 103:2452.
Davis, D.A. et al. (2005) Blood 105:4561.
Stumm, R.K. et al. (2002) J. Neurosci. 22:5865.
Veldkamp, C.T. et al. (2005) Protein Sci. 14:1071.
Charnaux, N. et al. (2005) FEBS J. 272:1937.
Percherancier, Y. et al. (2005) J. Biol. Chem. 280:9895.
Babcock, G.J. et al. (2003) J. Biol. Chem. 278:3378.
Klein, R.S. et al. (2004) Trends Immunol. 25:306.
Salcedo, R. and J.J. Oppenheim (2003) Microcirculation 10:359.
Broxmeyer, H.E. et al. (2003) J. Leukoc. Biol. 73:630.

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline)

Alternate Names

CXCL12/SDF-1 beta; hSDF-1beta; SDF 1beta; SDF1 beta; SDF1b

Related Research Areas

Citations for Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
Authors: J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann
Cell Stem Cell, 2021-12-25;29(2):217-231.e8.
Species: Human
Sample Types: Organoids
Applications: Bioassay
Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway
Authors: J Lim, J Heo, H Ju, JW Shin, Y Kim, S Lee, HY Yu, CM Ryu, H Yun, S Song, KS Hong, HM Chung, HR Kim, JS Roe, K Choi, IG Kim, EM Jeong, DM Shin
Sci Adv, 2020-04-15;6(16):eaba1334.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Stimulation of chondrocyte hypertrophy by chemokine stromal cell-derived factor 1 in the chondro-osseous junction during endochondral bone formation.
Authors: Wei L, Kanbe K, Lee M, Wei X, Pei M, Sun X, Terek R, Chen Q
Dev. Biol., 2010-03-04;341(1):236-45.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Applications: ELISA (Standard)
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Applications: ELISA (Standard)
A neurovascular niche for neurogenesis after stroke.
Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
J. Neurosci., 2006-12-13;26(50):13007-16.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
Int. J. Cancer, 2006-05-01;118(9):2147-57.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
Eur. J. Immunol., 2001-04-01;31(4):1037-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay