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Recombinant Human Ephrin-A3 Fc Chimera Protein, CF

Catalog #: 359-EA
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
359-EA-200
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ephrin-A3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A3 Fc Chimera (Catalog # BT359) for binding to immobilized recombinant mouse Eph-A6 Fc Chimera in a functional ELISA.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Ephrin-A3 protein
Human Ephrin-A3
(Asn31-Ser209)
Accession # AAA52368		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
AAA52368
N-terminal Sequence
Analysis
Asn31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47.7 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

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359-EA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

359-EA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A3

Ephrin‑A3, also known as EHK1‑L, EFL‑2, and LERK‑3, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑A3 preferentially interacts with receptors in the EphA family (1, 2). Ephrin‑A3 is an unusual Ephrin‑A molecule in its dependence on heparan sulfate binding for full activity (3). Mature human Ephrin‑A3 shares 92% aa sequence identity with mouse and rat Ephrin‑A3 (4). Its expression is restricted to discreet locations during the early development of multiple tissues (5). Ephrin‑A3 expression can be up‑ or down‑regulated by hypoxia in the hippocampus or vascular endothelial cells, respectively (6, 7). Ephrin‑A3 down‑regulation contributes to hypoxia‑induced endothelial cell chemotaxis, proliferation, and tubule formation (7). Its interaction with EphA receptors induces neurite growth cone collapse and the repulsion of migrating axons (8‑10). This activity is important for the accurate pathfinding of migrating axons during CNS development (10). Astrocyte‑expressed Ephrin‑A3 activates EphA4 on hippocampal neurons to regulate dendritic spine morphology and long term potentiation (8, 11, 12). The same interaction induces reverse signaling through Ephrin‑A3 to regulate glutamate uptake by the astrocyte and the availability of glutamate in the synapse (11, 12). Astrocyte‑expressed Ephrin‑A3 also interacts with EphA7 to inhibit the proliferation of neural progenitor cells (13).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Irie, F. et al. (2008) Proc. Natl. Acad. Sci. 105:12307.
  4. Davis, S. et al. (1994) Science 266:816.
  5. Duffy, S.L. et al. (2006) Gene Expr. Patterns 6:719.
  6. Pulkkinen, K. et al. (2008) FEBS Lett. 582:2397.
  7. Fasanaro, P. et al. (2008) J. Biol. Chem. 283:15878.
  8. Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
  9. Stein, E. et al. (1999) J. Neurosci. 19:8885.
  10. Rudolph, J. et al. (2010) Cell Adh. Migr. 4:400.
  11. Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
  12. Carmona, M.A. et al. (2009) Proc. Natl. Acad. Sci. 106:12524.
  13. Jiao, J. et al. (2008) Proc. Natl. Acad. Sci. 105:8778.
Entrez Gene IDs
1944 (Human)
Alternate Names
EFL2; EFL-2; EFNA3; Ehk1-L; EPH-related receptor tyrosine kinase ligand 3; EphrinA3; Ephrin-A3; EPLG3EHK1 ligand; LERK3; LERK3LERK-3; ligand of eph-related kinase 3

Citations for Recombinant Human Ephrin-A3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells
    Authors: YL Chen, YC Yen, CW Jang, SH Wang, HT Huang, CH Chen, JR Hsiao, JY Chang, YW Chen
    Scientific Reports, 2021-01-12;11(1):644.
    Species: Human
    Sample Types: Reference Standard
  2. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
    Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
    Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):.
    Species: Virus - Henipavirus
    Sample Types: Recombinant Protein
    Applications: Coprecipitation Assay
  3. Point mutations in dimerization motifs of the transmembrane domain stabilize active or inactive state of the EphA2 receptor tyrosine kinase.
    Authors: Sharonov G, Bocharov E, Kolosov P, Astapova M, Arseniev A, Feofanov A
    J Biol Chem, 2014-04-14;289(21):14955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Attenuation of eph receptor kinase activation in cancer cells by coexpressed ephrin ligands.
    Authors: Falivelli, Giulia, Lisabeth, Erika Ma, Rubio de la Torre, Elena, Perez-Tenorio, Gizeh, Tosato, Giovanna, Salvucci, Ombretta, Pasquale, Elena B
    PLoS ONE, 2013-11-29;8(11):e81445.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010-03-24;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Synaptic activity prompts gamma-secretase-mediated cleavage of EphA4 and dendritic spine formation.
    Authors: Inoue E, Deguchi-Tawarada M, Togawa A, Matsui C, Arita K, Katahira-Tayama S, Sato T, Yamauchi E, Oda Y, Takai Y
    J. Cell Biol., 2009-05-04;185(3):551-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Plasticity of neuron-glial interactions mediated by astrocytic EphARs.
    Authors: Nestor MW, Mok LP, Tulapurkar ME, Thompson SM
    J. Neurosci., 2007-11-21;27(47):12817-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
    Authors: Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
    Nat. Neurosci., 2003-02-01;6(2):153-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002-12-01;32(12):3745-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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