Recombinant Human EphB6 Fc Chimera Protein, CF Summary
Product Specifications
| Human EphB6 (Leu17-Ser579) Accession #AAI10608.2 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3384-B6
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: EphB6
EphB6, also known as Hep and Mep, is a 110 kDa member of the Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by ligand preference and have a common structural organization (1 - 4). The human EphB6 cDNA encodes a 1006 amino acid (aa) precursor that includes a 16 aa signal sequence, a 563 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 406 aa cytoplasmic domain. The ECD contains serine- and cysteine-rich regions and two fibronectin type-III domains. The cytoplasmic domain contains one non-catalytic protein kinase-like, one proline-rich, one SAM, and one PDZ-binding domain (5, 6). Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6. It shares 38 - 45% aa sequence identity with human EphB1, 2, 3, 4, and 6. Human EphB5 has not been characterized. Two secreted splice variants have been described in mouse but not in human (6). EphB6 is primarily expressed in brain, pancreas, thymus, and peripheral T cells (5, 7, 8). EphB6 forms stable heterodimers with EphB1 and participates in signal transduction by association with other enzymatically active molecules (9 - 11). Ephrin-B2 is the dominant ligand for EphB6, although Ephrin-B1 and Ephrin-B3 can also trigger responses (12 - 14). High concentrations of Ephrin-B2 inhibit cell adhesion and migration as well as tyrosine phosphorylation of EphB6. Conversely, low concentrations of Ephrin-B2 promote adhesion and migration and do not lead to EphB6 phosphorylation (15). The level of EphB6 expression is inversely correlated with tumor aggressiveness in a variety of malignancies (1). EphB6 also functions as a T cell co-stimulatory molecule (8, 11, 13). EphB6 clusters with the T cell receptor and participates in the subsequent attenuation of the T cell response (8, 10, 11, 13).
- Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
- Poliakov, A. et al. (2004) Dev. Cell 7:465.
- Wu, J. and H. Luo (2005) Curr. Opin. Hematol. 12:292.
- Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
- Matsuoka, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:487.
- Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.
- Hafner, C. et al. (2004) Clin. Chem. 50:490.
- Luo, H. et al. (2002) J. Clin. Invest. 110:1141.
- Freywald, A. et al. (2002) J. Biol. Chem. 277:3823.
- Freywald, A. et al. (2003) J. Biol. Chem. 278:10150.
- Luo, H. et al. (2001) J. Immunol. 167:1362.
- Munthe, E. et al. (2000) FEBS Lett. 466:169.
- Luo, H. et al. (2004) J. Clin. Invest. 114:1762.
- Shimoyama, M. et al. (2002) Biochem. Biophys. Res. Commun. 298:87.
- Matsuoka, H. et al. (2005) J. Biol. Chem. 280:29355.
Citation for Recombinant Human EphB6 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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EphB4 forward signalling regulates lymphatic valve development.
Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
Nat Commun, 2015-04-13;6(0):6625.
Species: Human
Sample Types: Antibody
Applications: Enzyme Assay
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