Recombinant Human EphB4 Fc Chimera Protein, CF

Catalog #: 11307-B4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11307-B4-100

Recombinant Human EphB4 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EphB4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human EphB4 Fc Chimera (Catalog # 11307-B4) is immobilized at 0.3 µg/mL (100 µL/well), Recombinant Mouse Ephrin-B2 Fc Chimera (Catalog # 496-EB) binds with an ED₅₀ of 0.600-7.20 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human EphB4 protein

Human EphB4 (Leu16-Arg539) Accession # AAH52804.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAH52804.1

N-terminal Sequence
Analysis

Leu16

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

58 kDa

SDS-PAGE

95-108 kDa, under reducing conditions.

Product Datasheets

11307-B4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11307-B4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human EphB4 Fc Chimera Protein (Catalog # 11307-B4) is immobilized at 0.3 µg/mL (100 µL/well), Recombinant Mouse Ephrin-B2 Fc Chimera Protein (496-EB) binds with an ED₅₀ of 0.600-7.20 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human EphB4 Fc Chimera Protein (Catalog # 11307-B4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-108 kDa and 190-220 kDa, respectively.

Reconstitution Calculator

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor tyrosine kinase family and binds Ephrin-B2. The A and B class Eph proteins have a common structural organization (1 - 4). The human EphB4 cDNA encodes a 987 amino acid precursor that includes a 15 amino acid (aa) signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 427 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. The ECD of human EphB4 shares 89% aa sequence identity with mouse EphB4 and 42 - 45% aa sequence identity with human EphB1, 2, and 3. EphB4 is expressed preferentially on venous endothelial cells (EC) and inhibits cell-cell adhesion, chemotaxis, and angiogenesis. Opposing effects are induced by signaling through Ephrin-B2 expressed on arterial EC: adhesion, endothelial cell migration, and vessel sprouting (6). EphB4 singaling contributes to new vascularization by guiding venous EC away from Ephrin-B2 expressing EC. Ephrin-B2 signaling induces arterial EC to migrate towards nascent EphB4 expressing vessels (6). The combination of forward signaling through EphB4 and reverse signaling through Ephrin-B2 promotes in vivo mammary tumor growth and tumor-associated angiogenesis (7). EphB4 promotes the differentiation of megakaryocytic and erythroid progenitors but not granulocytic or monocytic progenitors (8, 9).

References

Poliakov, A. et al. (2004) Dev. Cell 7:465.
Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
Bennett, B.D. et al. (1994) J. Biol. Chem. 269:14211.
Fuller, T. et al. (2003) J. Cell Sci. 116:2461.
Noren, N.K. et al. (2004) Proc. Natl. Acad. Sci. 101:5583.
Wang, Z. et al. (2002) Blood 99:2740.
Inada, T. et al. (1997) Blood 89:2757.

Long Name

Eph Receptor B4

Entrez Gene IDs

2050 (Human); 13846 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11